ISOLATION AND CHARACTERIZATION OF SECONDARY METABOLITES FROM THE ROOT CULTURE OF MORUS CATHAYANA

• Main Author: M. A. Hutasoit, Gabriel
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/75517
• Institution: Institut Teknologi Bandung

Plants are an essential part of human life and provide numerous benefits through naturally synthesized products. These benefits include both primary metabolites (as a primary food source) and secondary metabolites (used in pharmaceuticals, perfumes, food additives, dyes, etc.) that have a wide range of applications. Morus plants belong to the Moraceae family (mulberry family) and are extensively used in medicine due to their abundance of secondary metabolites, which play a crucial role in treatment. One such compound is 1-deoxynojirimycin (DNJ), isolated from Morus cathayana, known for its blood sugar-reducing properties. However, the diminishing cultivation land for plants, coupled with uncertain weather and climate conditions, has led to a decline in the availability of parent plants, making it increasingly challenging to obtain these important compounds from the plants. Therefore, tissue culture, a method of vegetative plant propagation by manipulating somatic plant tissues, has been studied and applied as an alternative source of secondary metabolites from parent plants. The hairy root culture of Morus plants has been reported to contain secondary metabolites, mainly of the Diels-Alder adduct type. In this study, secondary metabolites from Morus cathayana were isolated through the tissue culture of its roots. The roots of Morus cathayana shoots were grown using Murashige-Skoog (MS) agar medium and further multiplied using double-strength MS-2 liquid medium. The obtained hairy root culture was then macerated with ethyl acetate, followed by fractionation and purification using vacuum liquid chromatography (VLC), gravity column chromatography (GCC), and radial chromatography (RC). A total of 5.5 mg of yunanensin A was successfully isolated from the hairy root culture of Morus cathayana. Cytotoxicity test on P-388 murine leukemia cells showed that yunanensin A is very active with an IC50 value of 1,16 µg/mL.