MODIFIED CARBON PASTE ELECTRODES WITH MAGNETITE AND MOLECULAR IMPRINTED POLY(GLUTAMIC ACID) FOR VOLTAMMETRIC DETERMINATION OF METHYLENE BLUE

• Main Author: Faris Ansharullah, Ahmad
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/75565
• Institution: Institut Teknologi Bandung

The use of synthetic dyes in industry causes large amounts of non-biodegradable liquid organic dye waste and has the potential to cause environmental pollution. One of the dyes that is often used is glt blue. Disposal of high concentrations of methylene blue in water sources can reduce water quality and interfere with human health, such as causing eye irritation, skin irritation, and systemic effects including blood changes. According to the Decree of the Minister of the Environment through Kep-51/MENLH/10/1995 concerning liquid waste quality standards, this compound is only allowed to exist in the environment in relatively low concentrations, around 5-10 mg/L. In this research, a method for voltammetric analysis of Methylene blue was developed using a carbon paste electrode (EPK) modified by a Fe3O4 magnetic molecule and a polymer imprinted molecule with glutamic acid as the monomer. Synthesis of Fe3O4 was carried out and characterized using FTIR and SEM, then used in the modification of the working electrode with a polymer imprinted molecule. Molecular imprinted polymers were synthesized using cyclic voltammetry with a solution of analyte: monomer composition (1:3) with 15 cycles of electropolymerization. Methylene blue was measured using a square wave voltammetry (SWV) technique in 0.1 M PBS pH 6 at a potential range of -0.5 – 0 V with a scan rate of 125 mV/s. The measurement results of the modified carbon paste electrode had good repeatability and reproducibility with a linear working range at a concentration of 1-1000 ?M, and a limit detection is 1.136 ?M. Based on the scan rate analysis shows that the electron transfer process is controlled by the diffusion process. The determination of methylene blue by voltammetry and UV-Vis spectrophotometry in this study had a t value of 0.146. In addition, EPK- Fe3O4-MIP can also detect the presence of Methylene blue in fish blue drug samples. The results of the interference study indicated that there was interference from Pb2+ in the measurement of Methylene blue solution.