PRODUKSI LIPID DARI RHODOTORULA GLUTINIS MENGGUNAKAN LIMBAH PERKEBUNAN NIRA BATANG KELAPA SAWIT SEBAGAI MEDIA FERMENTASI

• Main Author: Hisyam Naufalino, Daffa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/75758
• Institution: Institut Teknologi Bandung

In Indonesia and Malaysia, palm oil plantation produces about 64-80 million palm trunk on average every year due to plant rejuvenation. The liquid squeezed from the palm trunk, called sap, has high sugar content, ranging from 9-17% (w/w) so it has the potential to be used as a carbon source for fermentation of high-value products. Rhodotorula glutinis is a yeast capable of synthesizing lipids in high amounts up to 63% of their dry weight. One strategy used to increase lipid production is nitrogen limitation. This study aimed to determine the effect of nitrogen addition on biomass consentration and lipid fraction in Rhodotorula glutinis cultivated in sap in batch mode for 120 days of cultivation. The study was conducted by varying the concentration of nitrogen sources (ammonium sulfate &; yeast extract: 0.25 &; 0; 0.88 &; 0.88; 1.76 &; 1.76, in g / L). The highest biomass consentration was obtained from treatment 3, which was 11.46 g / L, followed by treatment 2 of 7.82 g / L and treatment 3 of 7.28 g / L. The highest lipid consentration & lipid fraction was obtained from treatment 1 of 1.44 g / L and 21% (b / w), followed by treatment 3 of 0.43 g / L and 4.3% (b / w) and treatment 2 of 0.23 g / L and 3.4% (b / w). Based on the results of the study, the sap can support yeast growth even without the addition of large amounts of nitrogen. However, to increase the lipid content of cells, exploration of other strategies such as phosphate limitation strategies is needed.