PRODUKSI LIPID SEL TUNGGAL DARI RHODOTORULA GLUTINIS YANG DIKULTIVASI MENGGUNAKAN MEDIUM OIL PALM FROND JUICE (OPFJ) DENGAN VARIASI PENAMBAHAN KONSENTRASI NITROGEN
Production of oil from palm on an industrial scale produces large amounts of unused biomass, one of them is fronds. Oil palm fronds are known to have a high carbohydrate content in the form of simple sugars, which made up around 1-3% (g sugar/g fronds wet weight). The simple sugar content is obta...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/75761 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Production of oil from palm on an industrial scale produces large amounts of
unused biomass, one of them is fronds. Oil palm fronds are known to have a high
carbohydrate content in the form of simple sugars, which made up around 1-3% (g
sugar/g fronds wet weight). The simple sugar content is obtained through mechanical
pressing to produce oil palm frond juice (OPFJ). OPFJ has the potential to be used
as a fermentation medium, such as oleaginous yeast to produce single cell lipids.
Rhodotorula glutinis is a species that is known to accumulate high amounts of lipids.
The lipid content in Rhodotorula glutinis is dominated by unsaturated fatty acids,
hence it has potential as a raw material for the pharmaceutical industry. One of the
factors affecting the ability of Rhodotorula glutinis to accumulate lipids is nitrogen
limitation. This study aims to determine the effect of the addition of a nitrogen
concentration on biomass growth, substrate consumption profile, and lipid
accumulation. The highest biomass growth rate was obtained with the highest
nitrogen source addition (0.57 g/L yeast extract and 0.77 g/L (NH4)2SO4 (variation
1)) with a value of 0.29 hours-1 and a dry weight of biomass after 120 hours of
cultivation is 8.93 g/L. The highest substrate consumption rate was obtained in the
medium with the most abundant carbon and the lowest nitrogen source addition
(0.007 g/L yeast extract and 0.008 g/L (NH4)2SO4 (variation 3)) with a value of
0.032 g lipid/g biomass·hour. The highest yield of biomass to substrate was obtained
in variation 2 (0.12 g/L yeast extract and 0.17 g/L (NH4)2SO4) of 12.97 g/g at the
lowest carbon concentration. Based on the one-way ANOVA test and post-hoc test, it
was found that the addition of nitrogen had a significant effect on lipid content. The
highest content of lipid was achieved in conditions of nitrogen limitation (variation 3)
of 19.74% (g lipid/g dry biomass).
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