PRODUKSI MICROBIAL LIPID DARI RHODOTORULA GLUTINIS DENGAN VARIASI KONSENTRASI SUBSTRAT SUKROSA

• Main Author: Vania Pohan, Michelle
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/75762
• Institution: Institut Teknologi Bandung

Rhodotorula glutinis is a yeast with the ability to produce lipids up to 60,7% (w/w) of its dry biomass. The lipid accumulation by Rhodotorula glutinis can be stimulated under growth conditions with limited nitrogen concentration. In this study, Rhodotorula glutinis was cultivated in a nitrogen-limiting medium with varying excess carbon sources, specifically sucrose, at different initial concentrations. The purpose was to determine the effect of increasing the initial concentration of sucrose on the lipid yield from Rhodotorula glutinis. Three different concentrations of sucrose were used: 10 g/L, 20 g/L, and 40 g/L. The cultivation method used was batch culture, with an initial temperature of 30°C and an initial pH of 6,0. The biomass was harvested after 120 hours of cultivation. The highest lipid productivity was obtained with an initial sucrose concentration of 40 g/L, which was 0,118 ± 0,008 g/L/day. Meanwhile, the lipid productivities for sucrose concentrations of 20 g/L and 10 g/L were 0,060 and 0,028 ± 0,003 g/L/day, respectively. Based on the research results, it was found that increasing the initial sucrose concentration had a significant impact on enhancing the lipid yield from Rhodotorula glutinis, with a significance value of p < 0,01. Furthermore, increasing the sucrose concentration up to 40 g/L remained advantageous in improving both biomass and lipid yield, as indicated by the yield of biomass to substrate (YX/S) and the yield of lipid to substrate (YP/S), which were 0,218 g/g and 0,019 g/g, respectively.