PRODUCTION AND CHARACTERIZATION OF COMPLEX ECTOINE-CU2+ AS ANTI IRRITANT

• Main Author: Nayatri, Rai
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/75789
• Institution: Institut Teknologi Bandung

The skin is the largest organ of the body that functions as a protector from external disturbances, one of which is an irritant. Irritants are compounds that can damage the skin barrier and can be found in surfactants used in cosmetics. To prevent irritation, anti-irritating compounds are used. Ectoine is a compound that has anti-irritant properties because of its ability to protect skin proteins from binding directly with surfactants. Reacting ectoine with Cu2+ is expected to increase the anti-irritant properties because copper has been shown to have several benefits on the skin. The ectoine produced in this study came from Halomonas elongata BK-AG25 which was isolated from the Bledug Kuwu mud crater, Central Java. By synthesizing ectoine, the bacteria can survive extreme situations such as high salt concentrations. Ectoine was produced with 5% LB media and then incubated for 24 hours. The bacteria were then transferred to 5% MM63 media to optimize cell biomass and 18% MM63 media to optimize ectoine production. The media was then centrifuged and the cells in the pellet were transferred to 1% NaCl to diffuse the ectoine. Pure ectoine was then characterized using HPLC and FTIR with commercial ectoine as a comparison. For 50 ml of media, 0.187 +/- 0.02 grams of ectoine was obtained. To prepare the complex, ectoine is dissolved in NaOH solution to deprotonate the carboxylic group. After that, Cu2+ solution of Cu(II)nitrate trihydrate was added to this solution and heated at 100 oC for 6 hours. Ectoine-Cu2+ complex formed green in color, followed by FTIR characterization. From the FTIR characterization, it can be seen that the splitting energy of the asymmetric vibration and the symmetric vibration of carboxylates is 149 cm-1 where the carboxylates are likely to bind covalently bidentate with 2 different metals. The anti-irritant properties were then tested by looking at the solubility of the zein. Zein is a corn protein that is a model of the keratin protein in the skin. Zein will dissolve and denaturate in surfactant solutions but this solubility will be reduced in the presence of anti-irritating compounds. With the addition of ectoine, the percentage of denatured zein protein decreases, indicating that the ectoin produced has anti-irritating properties. At 0.5% of complex, the denatured zein protein was greater than 0.5% ectoine. However, the t-test stated that the two did not have a significant difference, so that it can be said that the complex did not affect the anti-irritant activity of ectoine.