PRODUCTION OF INTRACELLULAR, CELL-BOUND, AND EXTRACELLULAR POLYSACCHARIDES FROM PORPHYRIDIUM CRUENTUM: THE EFFECT OF ORGANIC CARBON SOURCES CONCENTRATION AND LIGHT INTENSITY

• Main Author: Shalsabila Salman, Nadya
• Format: Theses
• Language: Indonesia
• Subjects: Teknik kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/75852
• Institution: Institut Teknologi Bandung

<p align="justify">Polysaccharides are complex polymers of monosaccharides produced by living organisms. In microorganisms, polysaccharides can be produced by bacteria, fungi, microalgae, and cyanobacteria. In contrast to polysaccharides produced by bacteria and fungi, polysaccharides from microalgae generally contain methyl and sulfate groups which have various benefits in the nutraceutical, therapeutic, cosmetic and food industries due to their rheological and health properties. Porphyridium cruentum is one of the potential sulfated polysaccharides-producing microalgae. These polysaccharides can be found in the form of intracellular cellbound and extracellular polysaccharides (ICBPS and EPS). Mixotrophic cultivation mode employing organic carbon as a nutritional factor and light as an environmental factor, has proven effective in augmenting biomass growth and the accumulation of various high-value products such as lipid and pigment in microalgae. However, research related to the production of ICBPS and EPS under mixotrophic mode is still limited by its high production cost, particularly due to the high organic carbon such as glucose. Therefore, this study aims to investigate the impact of low-cost organic carbon sources concentration, using glycerol and tofu wastewater, as well as the effect of light intensity on biomass growth and ICBPS and EPS production in P. cruentum. Glycerol was varied at concentrations of 3 and 10 g L-1, while tofu wastewater was varied at concentrations of 5 and 10%-v v-1. The light intensity was varied at 52 and 88 ?mol m-2 s-1. The results of the study showed that the biomass growth and polysaccharides production increased in line with increasing concentrations of organic carbon and light intensity. The highest concentrations of biomass, ICBPS, and EPS were obtained at variations of glycerol 10 g L-1 and light intensity of 88 ?mol m-2 s-1 with yield of 14,13, 0,67 and 0,61 g L-1. P. cruentum also shows a good ability for wastewater treatment which is indicated by the reduction of COD up to 82,91%.