#TITLE_ALTERNATIVE#

• Main Author: Pertiwi, Dian
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/7624
• Institution: Institut Teknologi Bandung

Abstract : <br /> <br /> <br /> Radioimmunoassay (RIA) has become a good analytical technique for the diagnosis of thyroid function. The routine used of RIA technique is the liquid phase radioimnunoassay as separating methode, such as precipitation by polyethylene glycol. At present, the attention has been directed towards the solid phase radioimmunoassay technique. <br /> <br /> <br /> In the present study, the solid phase radioimmunoassay technique has been done by binding an antibody at the inner wall of the plastic tubes in which has been pre-coated with glutaraldehide. The antibody to be coated must be purified by affinity chromatography using protein A-sepharose as a colomn. Futher on, the titer of antibody has been determined by solid phase technique. Control has been conducted to each experimental parameter for obtaining optimal analytical results. The assay design is set up to the volume of standard solution, time and temperature of incubation to identify a sensitivity and to find the working range needed. <br /> <br /> <br /> From the data obtained, it is proved that by adding 20 ul T4 standard solution, 300 ul T4 - 125I in a glutaraldehide-coated tube and to be incubated for 24 hours at 25 C provides sufficient results in the working range of 14 - 780 nmol/l. The determination of characteristic undertaken are non specific binding (N5B), maximum binding (% Bo/T), concentration value at 50/ B/Bo, the value of control samples, coefficient of variance between assay of control samples and stability test.