TYROSINASE ENZYME INHIBITOR COMPOUNDS ISOLATION FROM ETHANOL EXTRACT OF MORINGA LEAVES (MORINGA OLEIFERA LAM.)

• Main Author: Rizkia Hardina Putri, Fannissa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/76562
• Institution: Institut Teknologi Bandung

Hyperpigmentation is a skin disorder that occurs when the increased of melanin production caused by the melanocyte and tyrosinase increasing activity. Hyperpigmentation can cause dark spots on the skin that can interfere with appearance. The compunds which contains tyrosinase enzyme inhibitor can reducing even vanishing hyperpigmentation. The result from the latest research before shows the moringa leaves ethanol extract potentially become as tyrosinase enzyme inhibitor. This research aimed to determine the potential of moringa leaves ethanol extract and fraction of moringa leaves as an inhibitor of tyrosinase enzyme and isolates the active compund. The extraction was carried out by maceration method with 96% ethanol solvent. Fractination was carried out using liquid-liquid extraction method with n-hexane solvent, ethyl acetate, and water as solvents. The tyrosinase enzyme inhibition test was carried out from the extract and fraction conducted with autobiographic TLC (qualitative) and IC50 value determination (quantitative). The IC50 values for the ethanol extract, n-hexane fraction, ethyl acetate fraction, water-ethanol, and kojic acid (control) fraction were 9,744.55 ± 814.20; 3,902.67 ± 40.29; 2,026.42 ± 58.71; 17,541.96 ± 790.17 ?g/mL. Fractionation was carried out on the ethyl acetate fraction with classical column chromatography method, because the ethyl acetate fraction showed the strongest inhibition of activity. The selected fractions were purified using classical column chromatography and preparative thin layer chromatography. The results of purification obtained isolate X, Y, and Z candidates. Isolate X candidate was continued for purity test was carried using single development TLC with three mobile phases and two-dimensional TLC. Isolate X was then characterized using a specific spray reagent and visualized using TLC with several flavonoid markers coumpounds as standard. Further characterization of isolate X was carried out using TLC-Densitometry to determine the UV spectrum of the isolate. From the characterization results, it can be concluded that isolate X is a compound from the flavonoid group, namely the astragalin which has an inhibition of the tyrosinase enzyme were 14.42% at a concentration of 500 ?g/mL.