EVALUATION OF ANTITUSSIVE AND EXPECTORANT ACTIVITIES FROM ETHANOL EXTRACT OF KARUK LEAVES (PIPER SARMENTOSUM ROXB.) ON MALE SWISS-WEBSTER MICE

• Main Author: Arta Uli Simanjuntak, Natalia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/76576
• Institution: Institut Teknologi Bandung

Coughing is a defense mechanism of the body to protect the respiratory tract and clear secretions such as mucus, foreign substances, and infectious organisms. The treatment of cough is categorized based on its type, with dry cough being treated with antitussive agents and productive cough with expectorants. Some communities use karuk leaves as a traditional remedy for cough. This research aimsto demonstrate the antitussive and expectorant activities of ethanol extract from karuk leaves. The testing was conducted on 5 test groups, namely the negative control group (0.5% CMC Na), positive control group (codeine 10 mg/kg body weight for antitussive and guaifenesin 26 mg/kg body weight for expectorant), and the test groups treated with ethanol extract from karuk leaves at doses of 87.5 mg/kg body weight, 175 mg/kg body weight, and 350 mg/kg body weight. Antitussive testing was performed by measuring the reduction in cough frequency and the increase in cough latency period in the test animals exposed to 7.5% citric acid aerosol. Expectorant testing was conducted by measuring the increase in red phenol concentration in the trachea of the test animals that were administered intraperitoneal red phenol as an indicator of mucus secretion. The observations of the test groups were compared with the negative control group. The test group treated with ethanol extract from karuk leaves exhibited antitussive activity at a dose of 350 mg/kg body weight, reducing cough frequency by 58.54% and increasing the latency period by 62.05%. Meanwhile, the test group treated with ethanol extract from karuk leaves showed expectorant activity at a dose of 175 mg/kg body weight, increasing the concentration of red phenol by 95.40%. Therefore, ethanol extract from karuk leaves can exhibit antitussive activity at a dose of 350 mg/kg body weight and expectorant activity at a dose of 175 mg/kg body weight.