FORMULA OPTIMIZATION OF PROPOLIS IMPREGNATED GAUZE

• Main Author: Stevanie
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/76595
• Institution: Institut Teknologi Bandung

he ability to protect wounds from the outside environment, prevent infection, and prevent excess fluid are important factors required for wound dressings used in wound healing. Propolis is a resin produced by bees that is collected by bees and mixed with beeswax as a nest-building material and a protective agent that has been extensively researched due to its antioxidant, anti-bacterial and antiseptic activities, so it is widely used for wound healing. Wound dressings generally do not provide antimicrobial and wound healing effects. Thus, in this study propolis was impregnated into the wound dressing. The aim of this study was to optimize the propolis-base formula to enhance the propolis impregnation into the gauze. The impregnated propolis in the gauze was evaluated by determining its antioxidant activity using DPPH method. In addition, characterization of gauze water vapor transmission was carried out using the ASTM (American Society for Testing Materials) E-96 cup method. Then, antimicrobial activity test was carried out using the inhibition zone and microdilution methods. The best propolis-impregnated gauze formulation in providing antioxidant activity was gauze containing liquid parafin: vaseline base (75:25), showing a DPPH inhibition of 21.105%, which was significantly different to the other two formulas and to untreated gauze. Transmission of water vapor in the formula with a ratio of liquid parafin: vaseline (75:25) showed the best transmission rate i.e 8.020 g/m2 hour. Propolis-impregnated gauze with concentrations of 25%, 30%, and 35% showed inhibition zones on S. aureus and P. aeruginosa but not for C. albicans. Microdilution test showed no antibacterial activity of propolis at concentration up to 2048 ppm. -- ---------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------------