ANALYTICAL METHOD DEVELOPMENT FOR THE DETERMINATION OF ASTRAGALIN IN MORINGA LEAVES EXTRACT (MORINGA OLEIFERA LAM.) USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

• Main Author: Rahima Putri, Fathia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/76600
• Institution: Institut Teknologi Bandung

Herbal medicine is frequently used in the community, so the safety and quality of raw materials and finished herbal medicinal products need to be monitored. Monitoring of herbal products can be done identifying and determining the level of marker compounds as specific parameters, therefore a validated analytical method is needed. This research was conducted to develop a validated High Performance Liquid Chromatography (HPLC) method that can be used to determine the level of astragalin in Moringa leaves extract. The optimum conditions were obtained using a LiChrospher® 100 RP-18 column (125 mm x 4 mm, 5 µm), mobile phase consisting of mixture of methanol and aquabides with gradient elution mode, flow rate of 1.0 mL/min, column oven temperature of 30 oC, and detection using UV detectors at 345 nm. The retention time of astragalin was 6.608 minutes with this system. The results of method validation showed good linearity, with correlation coefficient (R2 ) 0.991 and VX0 1.395%. the limits of detection and quantification of this method were 1.883 and 6.277 µg/mL, respectively. The accuracy test also met the requirements with the recoveries being 98.029–101.901%. Intraday and interday precision test gave a relative standard deviation less than 2% and Horwitz Ratio less than 2. The resolution value and selectivity factors of astragalin were 1.878 and 1.290, respectively. RSD value was less than 2% during robustness test by varying the flow rate, column oven temperature, and detection wavelength. The concentrations of astragalin in samples of Moringa leaves extract and herbal medicine were 1.081 and 0.009%, respectively. The results of this study showed that the developed HPLC method met all the acceptance criteria, therefore it can be used to determine astragalin in Moringa leaves extract.