DEVELOPMENT OF CONVENTIONAL TABLETS CONTAINING PROPOLIS NANOPARTICLE EXTRACT AS AN IMMUNOMODULATOR
The COVID-19 pandemic significantly affected the development of the pharmaceutical industry and the public demand for immunomodulators as substances that can modulate the immune system. Propolis is a natural material that holds potential as an immunomodulator due to its rich content of phenoli...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/76652 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The COVID-19 pandemic significantly affected the development of the pharmaceutical industry and
the public demand for immunomodulators as substances that can modulate the immune system.
Propolis is a natural material that holds potential as an immunomodulator due to its rich content
of phenolic compounds and flavonoids. However, due to the unfavorable properties of propolis and
the limited published studies, the use of propolis as an immunomodulator is still restricted. To
overcome its limitations in terms of pharmaceutical aspects, propolis extract in the form of nano
particles (ENP) has been developed. This study aimed to determine the characteristics of ENP
through phytochemical screening and particle size analysis using PSA and SEM, determine the
immunomodulatory activity of ENP with a dose of 5.04 mg/kg BW in wistar rats, and develop a
formulation of ENP in conventional tablet dosage form. The characteristics of ENP particles exhibit
irregular polygonal shape, with a size of 551 nm and a polydispersity index of 0.485 while also
positively containing phenolic compounds and flavonoids. ENP at a dose of 5.04 mg/kg BW
significantly increased the white blood cell count (323.16%) and lymphocyte count (268.78%) in the
blood on the 14th day compared to the control group (86.92%; 90.19%). In the spleen, ENP
increased the spleen index (0.48%), white pulp diameter (554.29 ?m), and lymphocyte count (129
cells) compared to the control group (0.43%; 483.26 ?m; 89 cells), although the differences were
not statistically significant. In the liver, the organ index significantly increased in the ENP group
(4.60%) compared to the control group (4.06%), and the abundance of mannose receptors in the
ENP group (17.15%) showed significant difference compared to the control group (5.27%). These
results indicate that ENP at a dose of 5.04 mg/kg BW exhibits immunostimulatory activity. Solid
dosage form of ENP with a composition of 5% PVP, 15% starch, 2% talc, 2% magnesium stearate,
and lactose as a filler resulted in a high-quality conventional tablet that meets all the requirements
of evaluation tests.
|
|---|