BIOSYNTHESIS OF ZINC OXIDE NANOPARTICLE USING PROPOLIS METHANOLIC EXTRACT AND EVALUATION OF ITS ANTIOXIDANT PROPERTIES

• Main Author: Ajeng Permata Dewi, Dwi
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/76964
• Institution: Institut Teknologi Bandung

Zinc oxide is one of metal oxide-based nanoparticles that attracted tremendous interest in wide fields application due to its unique structural and optical properties, good stability, and non-toxic properties. There are various methods for synthesizing zinc oxide nanoparticles (ZnO-NP), the physical and chemical methods are the most commonly used among them. Nonetheless, these methods are considered less environmentally friendly due to the requirements of high energy and pressure, along with the involvement of toxic chemicals usage in every processing stage. Therefore, biological methods have been emerged as an alternative to conventional physical and chemical methods. The biological methods are using biomolecules that are produced by organisms such as microorganisms, plant extracts and natural products to generate nanoparticles. The biological methods provide several advantages including simple, quick, cost-effective and ecofriendly. Propolis contains various bioactive compounds such as phytochemicals and proteins that can be served as reducing and stabilizing agents for the synthesis of metal nanoparticles. This study aimed to biosynthesize zinc oxide nanoparticles using methanolic extract of propolis coupled with the determination of nanoparticles activity as antioxidant. Propolis extract was characterized by qualitative phytochemical assay and LC-MS/MS analysis to identify the bioactive composition. The size, morphology, surface charge and crystallite structure of zinc oxide nanoparticles (Pro-ZnO NPs) were investigated using TEM (Transmission Electron Microscopy), zetasizer and XRD (X-ray diffraction). The presence of Pro- ZnO NPs and the possible functional groups responsible for Pro-ZnO NP fabrication were confirmed using UV-Vis spectrophotometer and FTIR analysis respectively. Antioxidant activity of Pro-ZnO NP was determined by DPPH (2,2- diphenyl-1-picrylhydrazyl) free radical scavenging assay. The characterization study obtained the presence of Pro-ZnO NPs characterized by the hexagonal shape with size range 30-50 nm and zeta potential -60.6 ± 0.78 mV. The XRD spectra proved the crystalline character of the Pro-ZnO NPs synthesized as hexagonal wurtzite with an average crystallite size of 30.04±5.53 nm. UV-Vis spectroscopy revealed a peak at 341 nm, which was the typical of ZnO NPs. The FTIR spectra illustrated the presence of possible functional groups present in ZnO NPs. The DPPH radical scavenging assay demonstrated the antioxidant activity of the nanoparticles (70.76 %) at a concentration of 100 mg/ml with IC50 value of 42.49 mg/ml. In conclusion, Pro-ZnO NPs was successfully synthesized using the propolis iv extract by biological method and the synthesized nanoparticles have shown promising activity as antioxidant.