ELISITASI ASAM SALISILAT UNTUK MENINGKATKAN KADAR ?-SITOSTEROL PADA AKAR ADVENTIF GINSENG JAWA (TALINUM PANICULATUM)

• Main Author: Soca Btarie, Rifca
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/76997
• Institution: Institut Teknologi Bandung

Cardiovascular disease especially hypercholesterolemia is a type of non-communicable disease is the highest cause of death. Reducing cholesterol levels in the body can be done by consuming ?-sitosterol, because ?-sitosterol can inhibit cholesterol absorption in the intestine. Alternative biomass that can be used as raw material for ?-sitosterol production is Talinum paniculatum. This statement is based on the adventitious roots of T. paniculatum or Javanese ginseng which contain ?-sitosterol. Even so, the levels of ?-sitosterol in the adventitious roots of Javanese ginseng are in relatively low amounts. Increasing the levels of ?-sitosterol can be done with growth environmental engineering like the use of the Nutrient Film Technique (NFT) hydroponic cultivation system and salicylic acid elicitation. Hydroponic NFT was used to increase root yield and elicitation was carried out to accumulate the production of ?-sitosterol compounds. Elicitation was carried out for 7 days for a total cultivation period of 28 days. In each cultivation period, salicylic acid was added with different concentrations of 0, 0.05, 0.1 and 0.2 mM. The effect of differences in concentrations of salicylic acid was observed through the dry weight of roots, shoot to root ratio, and ?-sitosterol levels. Based on the research, the results obtained are that the optimum concentration that can increase levels of ?-sitosterol is 0.05 mM. This concentration gave an increase in ?-sitosterol levels by 5.6 times to 1.871 ± 0.153 mg/g. Meanwhile, if we look at the root and shoot dry weight parameters, there is a negative correlation in the form of a decrease in root and shoot dry weight along with increasing elicitor concentration. Then, based on the root-to-shoot ratio parameter, the control treatment gave the lowest shoot-to-root ratio with a value of 4.95 (w/w).