FABRICATION OF SERICIN/CELLULOSE NANOFIBER-BASED HYDROGEL AS ALVEOLAR BONE SCAFFOLD CANDIDATE

• Main Author: Putri Anindita, Felicia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/77886
• Institution: Institut Teknologi Bandung

Attempts to repair alveolar bone defects are usually focused on regeneration of the bone tissue, which can be done by utilizing a porous scaffold. In this research, a hydrogel based on cellulose nanofiber (CNF) and sericin was successfully fabricated through freezedrying method as a biomaterial candidate for alveolar bone scaffold. CNF was isolated from banana peduncles using base hydrolysis, bleaching, and ultrasonication for 60 minutes. Results from Transmission Electron Microscope (TEM) and Fourier Transform Infrared Spectroscopy (FTIR) showed that the CNF with average diameter of 12.2 nm was synthesized. The CNF-sericin hydrogel was also made with freeze-drying with added variation of 0.1 grams and 0.25 grams of sericin (CNF-S10 and CNF-S25 respectively). After freeze-drying a cross-linking process using glutaraldehyde was added to increase stability of the scaffold. Scanning Electron Microscope (SEM) results showed that the CNF-sericin scaffolds had heterogeneous pore sizes (smaller pores of 12-48 ?m and bigger pores of 102-387 ?m) with interconnectivity that fulfills the requirements of an alveolar bone scaffold. Bioactivity of the scaffold was confirmed by the formation of an apatite layer at the surface of the hydrogels after immersion in Simulated Body Fluid (SBF) solution for 7 days. Overall characterization results showed the potential of CNF-sericin hydrogel to be used in alveolar bone scaffold applications.