EFFECT OF WHITE TEA (CAMELLIA SINENSIS (L.) KUNTZE) ETHANOL EXTRACT ON COL1A1 AND MMP1A GENE EXPRESSION IN UVA-IRRADIATED NIH/3T3 CELLS

• Main Author: Nur Ichwandini, Zahra
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78379
• Institution: Institut Teknologi Bandung

Collagen and Matrix Metalloproteinase (MMP) are proteins that has role in the skin aging process, especially in photoaging. The mechanisms of photoaging include the decreasing of COL1A1 gene expression and increasing of MMP1a gene expression due to UV exposure. This study was conducted to analyze changes in COL1A1 and MMP1a gene expression in UVA-induced NIH/3T3 cells due to administration of ethanol extract of white tea leaves (Camellia sinensis (L.) Kuntze) as the source of antioxidant. The primer efficiency test with qPCR gave a primer efficiency of COL1A1, MMP1a, and GAPDH of 104,89%, MMP1a of 137,65%, and GAPDH of 144,96% respectively. Cell viability test on ethanol extract of white tea leaves using prestoblue showed extracts at concentration of 50 ppm and 100 ppm are cytotoxic to NIH/3T3 cells. Primer specificity test using qPCR and gel electrophoresis showed that the primers COL1A1, MMP1a, and GAPDH were specific to produce PCR products with product lengths of 164, 172, and 87 base pairs, respectively. The ethanol extract of white tea leaves at concentration of 10 and 25 ppm for 6 hours UVA duration showed a pattern of increased COL1A1 mRNA expression that was not significantly different. UVA-induced cells for 6 hours showed a decrease in MMP1a mRNA expression, but not for 3 hours UVA duration. The ethanol extract of white tea leaves at concentration of 10 and 25 ppm at a UVA duration of 3 hours showed an indication of a pattern of decreased MMP1a mRNA expression. White tea ethanol extract has the potential to prevent photoaging.