CHARACTERIZATION AND ANTIFUNGAL ACTIVITY TEST OF CLOVE LEAF OIL (SYZYGIUM AROMATICUM (L.) MERR. & L.M. PERRY), ETHANOL EXTRACT, AND GUAVA LEAF FRACTION (PSIDIUM GUAJAVA L.) AGAINST TRICHOPHYTON RUBRUM

• Main Author: Azhar Husniah, Faridah
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78383
• Institution: Institut Teknologi Bandung

Trichophyton rubrum is a dermatophyte fungus that causes tinea pedis, a disease that infects the skin of the feet. Tinea pedis is common in tropical countries like Indonesia. Clove (Syzygium aromaticum (L.) Merr. & L.M. Perry) and guava (Psidium guajava L.) belong to the myrtaceae family, which are widely grown in Indonesia, and also have various pharmacological activities, including antifungal activities. This study aims to determine the potential of clove leaf oil (Syzygium aromaticum (L.) Merr. & L.M. Perry), ethanol extract, and guava leaf fraction (Psidium guajava L.) in inhibiting the growth of the fungus Trichophyton rubrum. Characterization parameters carried out on clove leaf oil samples referred to the parameters listed in the Standar Nasional Indonesia, and for crude drug and guava leaf extract referred to the Farmakope Herbal Indonesia. The antifungal activity test was carried out qualitatively using the disc diffusion method and quantitatively by determining the Minimum Inhibitory Concentration (MIC) and Minimum Fungicide Concentration (MFC) values using the microdilution method. The clove leaf oil MIC values was 0.48 µg/mL and its MFC was 0.96 µg/mL. The ethanol extract, ethyl acetate fraction, and water-ethanol fraction of guava leaves MIC values was 6250, 3125, and 3125 ?g/mL respectively. In conclusion, all test samples have antifungal activities against Trichophyton rubrum which clove leaf oil (Syzygium aromaticum (L.) Merr. & L.M. Perry) has excellent antifungal activity, whereas ethanol extract and guava leaf fraction have low antifungal activity.