ISOLATION OF ?- GLUKOSIDASE ENZYME INHIBITORS FROM TITANUS ( LEEA AEQUATA L)

• Main Author: Indrianto Bangar, Roy
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78401
• Institution: Institut Teknologi Bandung

Background and purpose: Diabetes mellitus is a metabolic disease characterized by high blood glucose levels in the blood. The World Health Organization stated that in 2025, the number of diabetics is estimated to be 333 million. The use of drugs based on natural ingredients can be an alternative treatment for diabetes with milder side effects and is expected to be more economical compared to conventional drugs. One of the plant families used to lower blood glucose levels is the Leeaceae family. One of the Leeaceae species currently used for traditional medicine is Leea aequata. In North Sumatra, this plant is known as titanus, and it is often used as traditional medicine by the local community. This study aims to isolate the active compound with alfa-glucosidase inhibitor activity from the titanus plant. Method: The processes began with collecting samples of leaves, fruit, and stems from the titanus plant that grows in North Sumatra. The extraction method used was maceration using 96% ethanol. The isolation stage starts with fractionation using the liquid-liquid extraction method, followed by column chromatography separation, each is monitored by TLC. Alfa-glucosidase was tested out on extracts, fractions, and isolates using a Multiscan microplate reader at ? 400 nm. The identification of compounds was carried out using UV-Vis and HNMR spectroscopy Result: The IC50 value of the ethyl acetate fraction was higher than that of the ethanol extract, n-hexane fraction, and water fraction, so it continued to the isolation stage. Two compounds (SF5 & SF6) were successfully isolated. The IC50 isolate against ?-glucosidase (SF5) was 12,25 ± 0,2 µg/mL and IC50 isolate (SF6) was 9,85 ± 0,8µg. The results of the suspected characterization of the SF5 isolate were 3,3?,4?,5,7-Pentahydroxyflavone 3-rhamnoside (Quercitrin) whereas the results of characterization SF6 were 3,3?,4?,5,7-Pentahydroxyflavone 3-?-glucoside (Isoquercitrin). Conclusion : From the results, it can be concluded that the active compound effect on ?-glucosidase is 3,3?,4?,5,7-Pentahydroxy flavone 3-rhamnoside (Quercitrin) and 3,3?,4?,5,7-Pentahydroxyflavone 3-?glucoside (Isoquercitrin).