ISOLATION OF THE MARKER COMPOUND OF PATCHOULI (POGOSTEMON CABLIN BENTH.) ESSENTIAL OIL

• Main Author: Putri Kamila, Shafina
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78424
• Institution: Institut Teknologi Bandung

Patchouli essential oil (POE) is Indonesia’s second most produced essential oil. This essential oil has a wide range of uses, especially in the cosmeceutical & perfumery field, because of its long-lasting fragrance. One of the parameters used to determine the quality of this oil is by looking at the percentage of its marker compound within the product, which is patchouli alcohol or Patchoulol. The aim of this research is to isolate & characterize a compound of the sample patchouli oil. The isolation is done through three processes, which is Vacuum Liquid Chromatography (VLC), done with the gradient method using a mixture of n-hexane-ethyl acetate as the mobile phase, open column chromatography, done with isocratic method using n-hexane as the mobile phase, & preparative thin layer chromatography (TLC), done using nhexane-ethyl acetate 20:1 as the mobile phase. The purity of the isolate was later tested by TLC with three different mobile phases & two dimensional TLC analysis. Based on its chromatogram, the isolate is found to have characteristics as such: it does not show any fluorescence under UV light, & appears as a spot with an Rf value of 0,61 (n-hexane-acetone 9:1 mobile phase) which has a purple color after sprayed with vanillin sulfate & anisaldehyde reagent & a brownish-orange color after sprayed with H2SO4 reagent. The isolate is proposed to be patchouli alcohol.