POTENTIAL OF BIOSURFACTANT PRODUCED BY BACILLUS LICHENIFORMIS F16 AS ANTIBIOFILM AGENT OF STREPTOCOCCUS MUTANS

• Main Author: Putri Luthfiah, Hanniyah
• Format: Final Project
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/78440
• Institution: Institut Teknologi Bandung

Data from the National Institutes of Health (NIH) shows that around 70% of microbial infections in the world are associated with the presence of biofilms. One of the bacteria associated with biofilm formation and causing human infections is Streptococcus mutans. This bacterium is one of the bacteria that form dental biofilm or dental plaque and acts as the main etiological agent for the formation of dental caries. Control and treatment of dental plaque can be done mechanically by brushing teeth using toothpaste and chemically using mouthwash. However, both contain synthetic surfactants that can have a negative impact on the environment and cause allergic reactions and irritation to certain skin or tissues. One alternative to synthetic surfactants that is more environmentally friendly is biosurfactants. Biosurfactants are known to have anti-adhesion and antibiofilm activities through their ability to change the hydrophobicity of the surface and reduce the interfacial tension between two different immiscible phases. This study aims to determine the type of biosurfactant produced by B. licheniformis F16 and its potential as an antibiofilm agent against S. mutans ATCC 25175 bacteria on glass substrate through anti-adhesion and biofilm eradication activities. The anti-adhesion and biofilm eradication activities of biosurfactants were evaluated based on biofilm wet weight loss, biofilm cell count and biofilm visualization with CLSM (Confocal Laser Scanning Microscopy) to determine MBIC (Minimum Biofilm Inhibition Concentration) and MBEC (Minimum Biofilm Eradication Concentration) values. FTIR and LC-MS results showed biosurfactant from isolate F16 is a surfactin type lipopeptide compound. Based on the MIC (Minimum Inhibitory Concentration) test, biosurfactant was able to inhibit the growth of planktonic S. mutans at a concentration of 15.63 ppm. Biosurfactant showed anti-adhesion and biofilm eradication activities with MBIC and MBEC values of 125 ppm and 250 ppm respectively. Visualization of biofilm cross-section with CLSM also showed inhibition of bacterial adhesion and biofilm eradication based on the decrease in biofilm biomass after biosurfactant treatment. This study shows that B. licheniformis F16 biosurfactant has potential as an antibiofilm agent against S. mutans bacteria.