FRACTINATION AND ANTIBACTERIAL ACTIVITY TEST OF SECONDARY METABOLIC FROM MARINE BACTERIA BACILLUS VELEZENSIS

• Main Author: Raudhatul Jannah, Zahra
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78443
• Institution: Institut Teknologi Bandung

Antibacterial resistance is a serious problem in the health sector. Due to antibacterial resistance, treatment of infection becomes difficult or even impossible. Especially for Gram-negative bacteria which are more easily resistant to antibacterials. Microbes themselves have been a source of antibacterial compounds that have been widely used clinically. Microbes originating from the sea are an alternative to finding new antibacterial compounds. Bacillus velezensis, a bacterium (code BPRBK1) from seawater from the collection of the Microbiology Laboratory of the ITB Pharmacy School showed activity against Eschericia coli. Therefore, the secondary metabolite of B. velezensis is expected to be an alternative as a new antibacterial which is active against Gram-negative bacteria. This research was conducted to obtain the secondary metabolite fraction of B. velezensis and determine its antibacterial activity against Gram-negative bacteria, namely E. coli, Salmonella typhi, and Pseudomonas aeruginosa. The research was carried out starting from the rejuvenation of B. velezensis, identification of B. velezensis, fermentation of B. velezensis, extraction, and fractionation of secondary metabolites from B. velezensis, and testing activity against Gram-negative bacteria. Extraction was carried out by maceration method for biomass and liquid-liquid extraction method for culture media with ethyl acetate solvent. The active extract was then fractionated using the Vacuum Liquid Chromatography (KCV) method. The activity test method used is the agar diffusion and microdilution method to obtain the Minimum Habat Concentration. The extracts and fractions obtained were then tested by microdilution to determine the minimum inhibitory concentration for the three test bacteria. The results showed that the media and biomass extracts from B. velezensis were active against all tested bacteria. The results of media extract fractionation obtained 4 fractions. There are 2 fractions (FB and FD) which are active against E. coli and P. aeruginosa with moderate-strong strength. There are also 2 fractions (FB and FC) which are active against S. typhi with moderate strength. The FB fraction of B. velezensis has potential as a source of Gram-negative antibacterial compounds.