EXPRESSION ANALYSIS OF GENE GROUPS RELATED TO GLYCOLYSIS & GLUCONEOGENESIS IN BACTERIAL METATRANSCRIPTOME FROM CAVENDISH BANANA (MUSA ACUMINATA, AAA)
Glycolysis and gluconeogenesis metabolism from endophytic bacteria are one of the factors that influence the ripening process of banana fruit. One effort to preserve fruit to prevent cooling is by coating chitosan as an edible layer. However, it is necessary to review further regarding the effect...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/78455 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Glycolysis and gluconeogenesis metabolism from endophytic bacteria are one of
the factors that influence the ripening process of banana fruit. One effort to preserve
fruit to prevent cooling is by coating chitosan as an edible layer. However, it is
necessary to review further regarding the effect of chitosan coating on the
distribution and activity of bacteria related to glycolysis metabolism and
gluconeogenesis in banana flesh during cooling. The aim of this research is to
determine the bacterial community in banana flesh with different ripening times
and their metabolic activities related to glycolysis and gluconeogenesis metabolism
through metatranscriptomic analysis. RNA-seq data was obtained from banana
flesh at temperature 1 and day 7, both in chitosan and control treatments. This
research method adapts the SAMSA2 pipeline. This method begins by filtering the
RNA sequence from rRNA to obtain mRNA data which is then annotated using the
NCBI RefSeq database and SEEDS Subsystem. The annotation results are then
annotated using the DESeq2 tool. Based on the results of the t test (p-value = 0.05)
on the bacterial consortium related to glycolysis & gluconeogenesis metabolism, it
was found that there was no significant difference in the number of bacteria between
the cooling and chitosan coating treatments. The bacterial profiles obtained in
various samples were the dominant bacterial phyla in the form of Proteobacteria
(42-51%), Firmicutes (12-15%), Cyanobacteria (11-25%), Actionobacteria (4-
10%), and Bacteroidetes (7-9%). %). From this bacterial consortium, the metabolic
activities of glycolysis and gluconeogenesis were found to be significantly different
for ripening, but not for chitosan coating based on the Wald test results (p-value =
0.05). When observing banana fruit that had undergone cooling, three groups of
genes were found to be regulated, namely 2,3-bisphosphoglycerate-independent
phosphoglycerate mutase (gpmI) which expressed the related bacterial phylum
Proteobacteria and Firmicutes, Pyruvate dehydrogenase E1 subunit alpha 1 (pdhA)
which expressed the phylum related bacteria Proteobacteria, and Pyruvate
phosphate dikinase (ppdK) which pours related bacterial phyla Proteobacteria,
Firmicutes, and Spirochaetae. There is also one gene that is included in the
upregulated group, namely Pyruvate decarboxylase (pdc) which is related to the
bacterial phyla Proteobacteria and Cyanobacteria. From the results of this study it
can be concluded that cooling and chitosan coating do not change the bacterial
consortium on bananas, however ripening can cause changes in 4 out of 45
differential gene expressions of -2 to 3.8 log2fold change related to glycolysis and
gluconeogenesis metabolism due to the interaction of bacteria with bananas.
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