ASSESSMENT OF THE PROTECTIVE EFFECT OF MITOCHONDRIAL- TARGETED QUERCETIN AGAINST ROTENONE-INDUCED MITOCHONDRIA DYSFUNCTION

• Main Author: Gabriella Alberta Fusen, Monica
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78458
• Institution: Institut Teknologi Bandung

Mitochondria is one of the crucial organelles in the body in maintaining normal body function. One of its function is producing energy in the form of ATP (adenosine triphosphate), it is also responsible for delivering signals that control cell’s proliferation and death. Mitochondria dysfunction will lead to decreased ATP synthesis, irregular cell death mechanisms, and increased ROS (Reactive Oxgen Species). Numerous diseases, including cancer, obesity, neurodegenerative disease, cardiovascular disease are frequently linked to increased ROS production. Therefore, to prevent oxidative stress from causing further damage, active antioxidant delivery systems are being developed with mitochondrial targeting. Previous study has developed nanoparticle formula containing quercetin as the antioxidant to protect cells from oxidative stress induced by H2O2. However, it was shown that H2O2 did not directly cause oxidative stress in mitochondria. As a result, this study used rotenone as a complex I inhibitor in the oxidative phosphorylation process to cause oxidative stress in mitochondria. LipoDQ with low dose (LD) of dequalinium (DQA) is utilized in this study considering that it has low toxicity profile towards cell. Based on the results of nanoparticle characterization, nanoparticles size <250 nm and polydispersity index of 0,5 was obtained. Furthermore, activity tests were done on TM4 cells that were induced by rotenone and stronger antioxidant activity was achieved in cells treated with lipoDQ LD using 0,5 ?M quercetin compared to rotenone control group and the group treated with liposomes alone. LipoDQ is proven to have significant effect on reducing the negative effect of rotenone-induced oxidative stress.