FORMULATION, EVALUATION SODIUM ASCORBYL PHOSPHATE NIOSOME IN GEL PREPARATION AND IN VITRO DIFFUSION

• Main Author: Desnita, Rise
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78540
• Institution: Institut Teknologi Bandung

Vitamin C used topically to prevent premature aging by antioxidant mechanism and as a precursor of collagen synthesis. Vitamin C has many benefits, but its disadvantage is unstable compound, especially on solution preparation and it is easily oxidized. Therefore, developed a derivative of vitamin C that is Sodium Ascorbyl Phosphate (NAF) with higher stability than vitamin C. The hydrophilicity of NAF is very high, so it has limitations in penetrating the lipid layer of the skin, therefore it needs a delivery system that can help improve the penetration capability of NAF. Niosome is a unilamellar or multilamelar vesicle system that entraps the active compound in layers made of nonionic surfactants and cholesterol. Niosome can improve stability of drug and penetration of the entrapped compound. It is expected the NAF in the form of niosome that is hydrophilic can enhance the penetration capability of NAF. Optimization of the niosom formulation composed of 100 ?mol of Span 60 by using thin layer hydration method and sonication to reduce the particle size. Furthermore, cholesterol and NAF concentrations was added to give the optimum entrapment efficiency. NAF entrapment efficiency was determined by separation method by using membrane dialysis. Then, Evaluation of particle size and morphology of niosome NAF was done. Niosome NAF was formulated in gel preparation with viskolam gel as a base. The stability of gel is tested at a temperature of 250C and 400C with the humidity of 75% . Diffusion test in vitro was tested using flow through and snake skin cell as membrane diffusion. Optimum niosome NAF was composed from Span 60 100 ?mol and 25 ?mol cholesterol. The concentration of NAF added was 3 % with entrapment efficiency was 96,09% ± 0,71 and had a particle size 144 nm ± 7,41. Niosome NAF formulation in gel preparation using 8% viskolam base the viscosity of 70367-82467 cps and pH of 6,30 to 6,36. The stability test showed that there was no significant difference between the level of niosome NAF and NAF in gel preparation. The diffusion test showed that niosome NAF gel can diffused 53,9% while the NAF gel diffused 26,25% for 8 hours. The stability of niosome NAF gel was not significantly different compared to the NAF gel stability. The formulations of NAF in niosome increased NAF diffusion 2 times better than the non niosome NAF formulated.