NON-INVASIVE ALTERNATIVE THERAPY OF ANEMIA USRâ¢.JG FE-DEXTRAN NANOPARTICLE
Fe-dextran is a complex consisted of ferryhydroxide and dextran. Intravenous administration of Fe-dextran is used in treatment of Fe deficiency causing anemia, chronic kidney disease, and increasing hemoglobin level in blood quickly. Limitation of intravenous administration are discomfort because of...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/78545 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Fe-dextran is a complex consisted of ferryhydroxide and dextran. Intravenous administration of Fe-dextran is used in treatment of Fe deficiency causing anemia, chronic kidney disease, and increasing hemoglobin level in blood quickly. Limitation of intravenous administration are discomfort because of skin tissue tearing, causing red sore on the skin due to injection, and sensitivity reaction (anaphylactic or anaphylactoid reactions). Disadvantage of oral administration of Fe-dextran is low therapeutic effectiveness because of slow absorption. Preparation of Fe-dextran nanoparücles was developed to improve bioavailability and to increase comfortable in use. Particles in nanosize increases the contact surface area so internalization of the particles into the lipid bilayer membrane in gastrointestinal tract would be more efficient. Nanoparticles were synthesized using the nanoprecipitation method by addmg ammonium in a variety of concentrations. Physical characterizauon of nanoparticles included size and size distribution of the particle, and zeta potential. Chemical characterization included entrapment efficiency and in vitro release study. The aim of bioavailability study of Fe-dextran nanoparticles in rat was to compare the pharmacokinetic parameters between Fe-dextran nanoparticles and ferrosulphate ms a control. The hemoglobin regeneration efficiency was also determined m the rat. As the results, Fe-dextran nanoparücles showed a panicle range 32.2-120.8 nm in size with a polydispersity index of 0.228-0.336, zeta potential of 0.02-0.20 mV and entrapment efficiency of approximately 81.14-90 04%. Release profile showed. that the Fe-dextran in HCI buffer pH I -2 was faster than that of phosphate buffer pH 6.8. Pharmacokinetic study result showed that AUC of Fe- dextran nanoparficles was the greatest comparing to that of ferrosulphate nanoparticles, fe dextran, and ferro sulphato (p<0.05). In addition, result of hemoglobin regeneration efficiency testing in rat showed that HRE (Hemoglobin Regeneration Efficiency) of the rat injected by fe dextran nanoparticles was greater than the rat injected ferrosulphate nanoparticles. Fe-dextran, ferro sulphate, and the rat which was not injected by Fe-dextran nanoparticles (p<0.05).
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