STABILITY AND IN VIVO STUDY OF BSA NANOEMULSION CONTAINING JACALIN AS TRANSCUTANEOUS AND SUBCUTANEOUS VACCINE DELIVERY SYSTEM
Modern approaches to vaccme such as subunit vaccines and recombinant proteins require adjuvant to compansate the loss of immunostimulatory activity of whole cell vaccines. Therefore, the major challenge of subunit vaccine is the fact that they show poor immunogenic effect. The objective of this rese...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/78547 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Modern approaches to vaccme such as subunit vaccines and recombinant proteins require adjuvant to compansate the loss of immunostimulatory activity of whole cell vaccines. Therefore, the major challenge of subunit vaccine is the fact that they show poor immunogenic effect. The objective of this research was to develop subcutanous and transcutaneous vaccine delivery system in the form of nanoemulsion. Bovine Serum Albumin (BSA) was used as a model protein antigen and jacalin as adjuvant. Jacalin is lectin isolated from jackfruit seed (Artocarpus integrifolia) known having the ability as a vaccine adjuvant while the oil in water nanoemulsion system can be used to control the release of antigen. Development of nanoemulsion containing jacalin was expected to induce a better Immune response. The proteins were incorporated into the oil phase as proteinlecithin solid dispersion. The optimum formula of nanoemulsion consisted of com oil 4.5%, Tween 80 15%, glycerin 15%, BSA solid dispersion 1%, and jacalin solid dispersion 0.25% with average globule size of 421.31 ± 20.2 nm. Stability tests show that nanoemulsion stable under storage condition at room temperature and 40C until 28 days. Stability of nanoemulsions were evaluated by measuring the globule size, polidispersity index, pH, and BSA entrapment efficiency. In vitro permeation study with combination using shed snake skin and rat skin showed that 97% BSA nanoemulsion was penetrated the stratum comeum and retained in the dermis. In vivo immunogenicity study showed that nanoemulsion BSA-jacalin formula provided the highest antibody titre when administered subcutaneously. Meanwhile nanoemulsion BSA formula provided the highest antibody titre when administered transcutaneously.
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