ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF ARBUTIN AND ITS DEGRADATION PRODUCTS IN COSMETICS CREAM USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

• Main Author: Chandrawati Anwar, Sofiyani
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78773
• Institution: Institut Teknologi Bandung

Arbutin (4-hydroxyphenol ß-D-glucopyranoside) currently widely used in whitening cosmetic. Arbutin could release hydroquinone which was prohibited substance used in cosmetic creams. In order to control the quality of products, an analytical method is required for analyzing arbutin and its degradation product simultaneously and ensuring safety of cosmetic products in market. The aim of this study was to develop and validate analytical methods for arbutin released in cosmetic cream products using high performance liquid chromatography (HPLC). HPLC assay has been developed for arbutin and its degradation. The mobile phase used was a mixture of methanol-water (20:80) with flow rate 1 ml / min, 20 PI-J volume injector and detector PDA-UV, wavelength 284 nm, column Zorbax Eclipse Plus@ C18 5 gm (4.6 x 250 mm) temperature of 300C. The method was successfully validated with the following parameters: selectivity, linearity, limit of detection (LOD), limit of quantitation (LOQ), precision and accuracy. Selectivity shown by the similarity factor of arbutin and hydroquinone were at 995.20 and 997.89 and resolution (Rs) was 8.46. A calibration curve was linear in the range of 1.03 mg/mL — 7.20 mg/mL for arbutin and 0.99 mg/mL 6.98 mg/mL for hydroquinone, linear regression equation obtained for arbutin was y = 156.6708x 4.0850 with a correlation coefficient r 0.9999 and for hydroquinone was y — 435.4467x — 42.8450 with r — 0.9999. LOD and LOQ of statistical calculations were found 0.077 mg/mL and 0.23 mg/mL or 0.18 mg/g and 0.54 mg/g for arbutin respectively and hydroquinone were found 0.11 mg/mL and 0.34 mg/mL or 0.28 mg/g and 0.85 mg/g respectively. Signal to noise ratio approaches LOQ for arbutin were found 0.14 pg/mL or 0.27 pg/g and hydroquinone were found 3.23 gg/mL or 6.47 pg/g respectively. Intra-day precision parameter results for three days for arbutin respectively 0.83%, 1.71% and 0.70% and hydroquinone were 1.90%, 1.45% and 1.50%. whereas inter-day precision was 0.25% for arbutin and hydroquinone 1.59%. The average percentage of recovery was 97.70% for arbutin and 91.01% for hydroquinone. Degradation of arbutin was performed using HCI 1M and showed that arbutin released hydroquinone in acidic conditions. The method of analysis has been applied to the assay of eight products on the market.