ISOLATION AND FUNGI ISOLATE TEST OF ANTIMICROBIAL ACTIVITY MARINE ALGAE IN SOME SPECIES OF THE MARINE KABUPATEN TAKALAR, SOUTH SULAWESI

• Main Author: Fitriana
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78832
• Institution: Institut Teknologi Bandung

Background and Purpose: Marine microorganisms especially the fungi, have became important sources to obtain new compounds with potential pharmacological activity. Marine fungi associated with a wide variety of substrates, including sponges, mangroves, algaes and tunikats. Secondary metabolites of fungi derived from marine algae is reported to have the potential to be developed as antimicrobials such as antibacterial, antifungal, antiviral and etc. The purpose of this study was to obtain marine fungal strains with potential biological activity as an antimicrobial. Methods: Isolation of fungi from seven species of algae samples using Agar YPD medium which are the composed by yeast extract, peptone, dextrose and agar with compositlön 16 in artificial sea water and added to the antibiotic streptomycin sulfate, incubated for 5-7 days at incubator. Against the growing fungal colonies transferred to the new media to obtain pure fungi isolates. Pure fungal isolates fermented in broth YPD medium for 3 weeks. Liquid culture and fungal mycelia separated by filtration. Liquid culture extracted by liquid-liquid extraction while the mycelia extracted by maceration for 24 hours with solvent ethyl acetate. The solvent was evaporated to obtain dry extracts and tested their antimicrobial activity against Escherichia coli, Bacillus subtilis and fungi Candida albicans by disc diffusion method. Extracts which have antimicrobial activity were then determined the minimum inhibitory concentration using microdilution method and minimum kill concentration in Mueller-Hinton agar medium. Results: 18 pure fungi isolates obtained from seven species of algae samples. The antimicrobial activity ofthe extract liquid culture shown by extracts A2, A3, A4, C3, C4, D, Gl, G2 with MIC and MBC 1024 gg/mL, extract F3 with MIC and MBC 512 gg/mL of the bacteria Escherichia coli and extract A4 , B, C3, C6, F3 with MIC and MBC 1024 gg/mL of the the bacteria Bacillus subtilis. The antimicrobial activity of the extract of the mycelia shown by extracts A3, B, E, F3 with MIC and MBC 1024 gg/mL of the Escherichia coli and extracts Al, A2, A4, C3, C4, D, F2, F3 with MIC and MBC 1024 gg/mL an&vxtract E with MIC and MBC 512 gg/mL of the bacteria Bacillus subtilis. While the extract of liquid culture and extract of mycelia of the fungus Candida albicans have no antimicrobial activity. Conclusion: The antimicrobial activity shown by extracts from a liquid culture F3 of the sample AKT.F (red algae) that gives MIC and MBC values of 512 mg / mL of the bacteria Escherichia coli, while extracts from mycelia E of samples AKT.E (red algae) which gives MIC and MBC values of 512 mg/mL ofthe bacteria Bacillus subtilis. Therefore extract liquid culture F3 and mycelia extract E are potential as an antimicrobial.