OPTIMUM CONDITION DETERMINATION AND DEVELOPMENT OF PORPHYRIN DERIVATED AS REAGENT FOR LEAD METAL ANALYSIS
Lead is one of the most widely distributed heavy metals in the environment. Exposure to and absorption of lead is still a problem because it can contaminate food and water. One of the most common methods used for the analysis of lead uses atomic absorption spectrophotometry (AAS). However, the use o...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/78842 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Lead is one of the most widely distributed heavy metals in the environment. Exposure to and absorption of lead is still a problem because it can contaminate food and water. One of the most common methods used for the analysis of lead uses atomic absorption spectrophotometry (AAS). However, the use of AAS is relatively expensive. UV-Vis spectrophotometry is an alternative instrument that can be used in metal analysis because it is easy to handle and relatively low cost. meso-tetrakis(N-methylpyridinium-4-yl) porphyrin (TMPyP) is a porphyrin derivative that has an absorption spectrum in the visible light region (400-800 nm), and can form metal complexes. The purpose of the present study is to obtain the optimum condition of complex formation of Pb-fi1PyP, and use TMPyP as an analytical reagent using UV-Vis spectrophotometry. This study began with finding the optimum condition of the complex formation of Pb-TMPyP by varying parameters such as pH, reaction time, temperature, and concentration, followed by the development and validation of analytical methods of lead (linierity, specificity, limit of detection, limit of quantification, sensitivity, accuracy, and precision) using TMPyP as an analytical reagent. The results of the optimization of the reaction showed that the optimum conditions for complex formation of TMPyP-Pb are at room temperature, a pH of 11.6, with a measuring time of 15 minutes. Validation of the analytical method showed that this method has a coefficient of correlation of 0.9959, LOD value of 0.05 ug/ml, LOQ value of 0.17 gg/ml, average molar absorptivity of 1.450k 105 M-1 cm-I, percentage of recovery 94-110%, and coefficient of variation for interday and intraday 3.38%, 3.74%, respectively. The specificity of foreign ions are < 3 gg for Hg, < 0.2 gg for Zn, and < 5 pg for Ca and As. The optimum reaction conditions for the formation of the complex Pb-TMPyP are at room temperature, a pH of 11.6, with a measuring time of 15 minutes, and this method can be applied prospectively for analyzing trace amounts of lead using UV-Vis spectrophotometry.
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