ANTIBACTERIAL ACTIVITY STUDY OF LACTOBACILLUS AND COMBINATION OF BACTERIOCINS WITH STANDARD ANTIBIOTICS AGAINS RESISTANT BACTERIA

• Main Author: Venita Kusumaningrum, Laurentia
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78884
• Institution: Institut Teknologi Bandung

Background and purpose: The incidence of nosocomial infections increased due to an increment of resistant bacterial strains. Methicillin resistant Staphylococcus aureus (MRSA), coagulase-negative staphylococci, and strains of multi-drug-resistant Streptococcus pneumoniae and Pseudomonas aeruginosa are the most resistant bacteria that caused nosocomial infection. This study evaluated antibacterial activity of supernatant of Lactobacillus brevis FNCC 0021, Lactobacillus delbrueckii FNCC 0045, Lactobacillus plantarum FNCC 0027, Lactobacillus ramnosus FNCC 0052, bacteriocins of L. brevis and combination of bacteriocins with standard antibiotics against nosocomial-infection-causing-resisiant-bacteria Pseudomonas aeruginosa Multidrug Resistant (MDR), MRSA, and Methicillin Resistant Coagulase Negative Staphylococcus (NfRCNS). Methods: Antibacterial activity ofLactobacillus supernatant, bacteriocins and combination bacteriocins with standard antibiotics were tested by agar diffusion method to determine inhibitory area and microdilution method to determine the Minimum Inhibitory Concentration (IIIC) and Minimum Bactericidal Concentration (MBC). Sample test with the highest inhibitory activity got partial purification to obtain a pure bacteriocins. Purified bacteriocins and the combination of purified bacteriocins with standard antibiotics (vancomycin, meropenem, tetracycline and ciprofloxacin) were tested for antibacterial activity. Results: The supernatant culture of L. brevis, L. delbrueckii, L. plantarum, and L. rhamnosus had antibacterial activity against resistant bacteria. L. brevis supernatant had a relatively greatest of the antibacterial activity against to Gram-negative bacteria than the other supernatant cultures. The diameter of inhibition zone ofL. brevis against P. aeruginosa MDR, hfRSA, and IIRCNS respectively were 11.2±0.15 mm, 12.0±0.23 mm and 11.8 mm. WC and WC L. brevis supernatant against P. aeruginosa MDR and MRSA were respectively 6.25% and 12.5%. Furthermore MBC, MIC of L. brevis supernatant against MRCNS gave similar value, which was 6.25%. Diameter of inhibition zone of L. brevis bacteriocins compared to L. brevis supernatant against P. aeruginosæ MDR were respectively 14.7±1.11 mm and 11.2±0.65 mm. The combination of bacteriocins with standard antibiotics against resistant bacteria increased MC level. Conclusion: The supernatant culture of L. brevis, L. delbrueckii, L. plantarum, and L. rhamnosus have antibacterial activity against P. aeruginosa ADR, MRSA and 1VfRCNS. Supernatant ofL. brevis shows the highest antibacterial activity against Gram-negative bacteria than the other supernatants. Bacteriocins have a role in the antibacterial activity of supernatants L. brevis. Interaction between L. brevis bacteriocin with meropenem, tetracycline and ciprofloxacin against resistant bacteria are antagonist.