THE EFFECT BINAHONG ETHANOLIC EXTRACT (ANREDERA CORDIFOLIA} (TEN) V. STEENIS.) IN LIPID PROFILE OF WISTAR RAT INDUCED HIGH FAT DIET

• Main Author: Abdul Rahman, Asep
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78900
• Institution: Institut Teknologi Bandung

The high level of cholesterol serum, primarily low-density lipoprotein (LDL) cholesterol in the blood will initiate the formation ateroma plaque. This condition will increase the risk of occurrence of cardiovascular disease such as coronary artery disease and stroke. Binahong leaves empirically have been used to reduce cholesterol levels. In previous studies, it has been shown that Binahong leaves have an activity in lowering cholesterol levels on hypercholesterolemic animal. In this study, activity of ethanol extracts of Binahong leaves will be tested in inhibiting the increase of cholesterol levels on the animals fed with a highcholesterol chow. Animal model established by hypercholesterolemic approach with induction using high-cholesterol chow, pure cholesterol, cholic acid, and propiltiourasil which were administered orally. The animals were divided into 5 groups, including the positive control group, ethanol extract of binahong leaves at doses of 50 mg/kg bw, 100 mg/kg bw, 200 mg/kg bw and simvastatin 36 mg/kg bw. Extract were given at the same time as hypercholesterol induction. Lipid profile parameters such as total cholesterol, triglycerides, and HDL-cholesterol were measured at the time before the induction, 15 days and 29 days after induction. LDL cholesterol level was calculated using Friedewald formula. While index of atherogenic was the log form of the triglyceride levels against HDL. At the end of the treatment, test animals were sacrificed and part of the aorta was isolated for identification of thickening of the wall of the aorta. The results showed that level of cholesterol and LDL of extract binahong at dose of 50 mg/kg (respectively 109.17±26.60 mg/dL and 55.78±23.92 mg/dL) had significant difference compared to the positive control group (144.00±33.62 mg/dL for cholesterol and 90.83±47.12 mg/dL for LDL). There was no significant difference on level of trygliceride and HDL of binahong extract' at a dose of 50 mg/kg rat compared to positive control. However, binahong extract at dose of 50 mg/kg bb had the lowest atherogenic index (0.68±0.27) compared to other extracts on day 15 of induction. The aorta thickness of binahong extract at a dose of 50 mg/kg bw rat group (150.60±41.72 pm) was lower than the positive control group (184.90±144.91 pm). In conclusion, extract binahong at dose of 50 mg/kg bw rat gives the best effect to prevent the increase of total cholesterol and LDL level on wistar male rats fed with a high-cholesterol chow.