ANTIOXIDANT ACTIVITIES OF LEAVES EXTRACTS FROM FOUR ARDIIS. SP AND THEIS CORRELATIONS WITH TOTAL FLAVANOID, PHENOLIC AND COROTENOID CONTENT

• Main Author: Budiana, Wempi
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78917
• Institution: Institut Teknologi Bandung

Free radicals are compound or molecule which have one or more unpaired electrons. Free radical have correlation with degenerative diseases. Leaves of Ardisia sp contain flavonoid and phenolic compounds. Some reasearches revealed that phenolic and flavonoid compounds could be correlated with antioxidant activities. The objectives of this research were to determine the antioxidant activity of various leaves extracts from four Ardia sp by determining IC50 of DPPH scavenging activity and EC50 of CUPRAC capacity, total phenolic, flavonoid and carotenoid content, their correlation with the IC5() DPPH and EC50 CUPRAC, and correlation between IC50 DPPH and EC50 CUPRAC. Extraction was performed by reflux apparatus using three solvents with increasing polarity, namely n-hexane, ethyl acetate and ethanol. Extracts were monitored by thin layer chromatography (TLC). DPPH, EC5() CUPRAC, total phenolic, flavonoid, carotenoid content of each extract was conducted by ultraviolet-visible spectrophotometry. Correlation between total phenolic, flavonoid, carotenoid content with IC50 DPPH and EC50 CUPRAC, correlation between ICso DPPH and EC50 CUPRAC were analyzed by using Pearson's method. Lowest antioxidant activity by DPPH method was given by ethanol leaves extract of Ardisia crenata (IC50 0.49 gg/mL), while the lowest antioxidant capacity by CUPRAC method (EC50 30.34 gg/mL) was given by ethyl acetate leaves extract of Ardisia eliptica. Ethanol leaves extract of Ardisia eliptica had the highest total phenolic (29.54 g GAE/IOO g), ethyl acetate leaves extract of Ardisia cymosa showed the highest total flavonoid (8.24 g QE/IOO g) and n-hexane leaves extract of Ardisia fuliginosa gave the highest total carotenoid (13.37 g BE/100 g). Total phenolic content in leaves extract of A. crenata, A- cymosa, A. fuliginosa had high, negative and significant correlation with IC50 DPPH (r -0.748, p<O.01•, r = -0.772, p<0.01; r -0.762, p<0.01). Total flavonoid content in all of leaves extracts of Ardisia sp had negative, high and significant con-elation with EC50 CUPRAC. IC50 DPPH of Ardisia crenata leaves extracts had positive, high and significant correlation with their EC5() CUPRAC (r = 0.967, p<0.01). Ali of leaves extracts of Ardisia sp can be categorized as very strong antioxidant. Phenolic compounds in leaves extracts of A. crenata, A. cymosa, A. fuliginosa were the mayor contributor in antioxidant activities by DPPH method, while flavonoid compounds in all of leaves extract of Ardia sp were the major contributor in antioxidant capacities by CUPRAC method. DPPH and CUPRAC methods gave linear result in antioxidant activity ofA. crenata leaves extracts.