ISOLATION OF SINENSETIN AND OTHER COMPOUNDS FROM {ORTHOSIPHON STAMINEUS}, BENTH
Kumis kucing (Orthosiphon stamineus,Benth.) is one of medicinal plants that has been used traditionally since long time ago. Several traditional use of this plant have been also proved by scientific researches, so that this plant has been developed from traditional use to rational phytotherapy with...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/78923 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Kumis kucing (Orthosiphon stamineus,Benth.) is one of medicinal plants that has been used traditionally since long time ago. Several traditional use of this plant have been also proved by scientific researches, so that this plant has been developed from traditional use to rational phytotherapy with some indications. Kumis kucing contains sinensetin that has pharmacological activities as antioxidant, antibacterial, and diuretic. Sinensetin is a marker compound of kumis kucing plant for standardization of herbal medicines containing this plant. Since kumis kucing has many pharmacological activities, it is assumed that the compounds which are responsible for the activities not only sinensetin, so that the other compounds could probably be a marker compound of this plant. Sinensetin and other compounds from kumis kucing are expensive and rare found in Indonesia, therefore there is a need to isolate these compounds and optimize the isolation procedures. Extraction was done using Soxhlet apparatus with ethyl acetate as a solvent. Ethyl acetate extract of kumis kucing (Orthosiphon stamineus, Benth.) was separated with liquid-liquid extraction using CuC12 and NaOH solution to clean up the chlorophyll contents. Subfractination was performed by vacuum liquid chromatography (VLC), radial thin layer chromatography, column chromatography and TLC preparatif Isolate was characterized by UV-Vis spectrophotometry and Nuclear Magnetic (NNfR)- 1H. Based on UV-Visible spectrum, isolate X had Xmax at 267 and 319 nm. After adding shift reagens, there did not give bathochromic or hipsochromic. Based on NB,'IR- IH spectrum, isolate X had chemical shift at 3,82; 3,88; 3,90; 4,01,6,55; 7,10 and 7,99 ppm, Isolate Y had Xmax at 269 nm and 328 nm. Isolate X was supposed to be 5,7,8,4'-tetramethoxy flavone which have one free OH group in ring A or ring B and isolat Y was supposed to be sinensetin. |
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