THE USE OF (MORINGA OLEIFERA} LAM.) LEAF EXTRACT AS ADJUVANT FOR DELIVERY SYSTEM OF HBSAG-LOADED NANOPARTICLE

• Main Author: Ahmadi, Adik
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78932
• Institution: Institut Teknologi Bandung

Recombinant antigent for the use of vaccine is often less immunogenic than live attenuated or inactive vaccine. Hence, a potent adjuvant is needed to enhance immune response. Moreover, the role of vector design is also important to facilitate the improvement of immune response. The aim of this reasearch was to develop HBsAg-loaded nanoparticles and Moringa oleifera aqueous leaf extract as adjuvant by using chitosan polymer. Chitosan nanoparticle was prepared by ionic gelation method using sodium tripolyphosphate as cross-linking agent. A system was composed of chitosan core in which HBsAg and M. oleifera extract were incorporated. Concentration of HBsAg used in this combination was 10 gg/mL and concentrations of extract were 10, 50 and 100 ug/mL, respectively. In this study, three types nanoparticles were produced, HBsAg-loaded nanoparticles, M. oleifera-loaded nanoparticles and combination HBsAg-M. oleifera loaded nanoparticles. The nanoparticles formed were characterized for panicle size, HBsAg entrapment eficiency using SDS-PAGE (sodium dodecyl sulfate — polyacrylamide gel electrophoresis) and extract entrapment efficiency based on total flavonoid content. To evaluate the effectiveness of the nanoparticles in stimulating immune response, dendritic cell maturation study was carried out. The results showed that particles size were between 111-245 nm. Entrapment efficiency of HBsAg in separated formula was 79,47% while that in combined formula were approximately 96-99%. Futthermore, entrapment efficiency of extract in separated formula were around 64-91 % while that in combined formula were 55-83%. Nanoparticles HBSAg-extract in combination gave better immune response than separated nanoparticles, while the highest immune response in order to mature dendritic cells was given by nanoparficles containing combination of HBSAg 10 gg/mL and extract 10 gg/mL. Nanoparticles can induce immune response but not as much as standarsd HBsAg. However, the use of nanoparticle is expected to be more efficient for oral administration.