FORMULATION AND ACTIVITY TEST OF CINCHONINE NIOSOMES AS HAIR GROWTH STIMULANTS

• Main Author: Hariyanti
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78947
• Institution: Institut Teknologi Bandung

Background and objectives: Cinchonine is the active ingredient taken from the isolation process of ethanol extract Cinchona Succirubra (bark) which is considered to have activity as stimulant of hair growth. Cinchonine is practically insoluble in water, slightly soluble in chloroform and alcohol so that it gives impact toward low transfollicular penetration of cinchonine into dermal papilla, only hydrophilic of active ingredient can through hair follicle and reach in dermal papilla. Thus, it requires delivery system that can decrease of cinchonine hydrophobicity to improve the cinchonine penetration into dermal papilla. Niosomes is a vesicle with double-layer structure produced from hidration of combination nonionic surfactant and cholesterol, able to decrease of cinchonine hydrophobicity. The aim of this study was formulation and activity test of cinchonine niosomes as hair growth stimulans. Method: Formulation of cinchonine niosomes began with the gel—liquid transition temperature (Tg) of Span 60 by Differential Scanning Calorimetry (DSC). Then it continued with the optimation process and formula of niosomes including the optimation of rotavapor velocity at the forming of thin-layer film, thé hydration temperature, the rotavapor velocity at hydration, hydration time, sonication time, the number of Span 60, cinchonine, and cholesterol. The optimal evhuation of niosomes formula could be seen from niosomes characteristic, which were among others the size of particle and polidispersity index by using Particle Size Analized (PSA), and the entrapment efficiency of cinchonine by using HPLC. The stability test was carried out for 28 days with the parameter size of particle, Polydispersity index, and the efficiency entrapment of cinchonine. In vivo test was carried out including the irritation test and the activity test of cinchonine niosomes for 14 days, with The parameter of activity test included the length of hair, the amount of hair per cm2 compared to control. Result: Gel—liquid transition temperature (Tg) of Span 60 was 45±20C, rotavapor velocity in the forming of thin-layer film of niosomes 210 rpm, temperature of hydration 55±2 0C, rotavapor velocity of hydration 210 rpm, hydration time 20 minute, sonication time of niosomes suspension I minute. The number of Span 60 100 gmol, cinchonine 0,03%b/v and cholesterol 15% from Span 60, with the entrapment efficiency •of cinchonine niosomes was The suspension of cinchonine niosomes showed that the good stability was in the storage at the room temperature (250C) and the storage at the temperature of 400 C, 75% RH for 28 days, with parameter: size of particle are 200 - 400 nm, Polydispersity index are 0,2 - 0,5, and the entrapment efficiency of cinchonine are 83 - 85%. The iritation test of the suspension of cinchonine niosomes at l, 24, 48 and 72 hour resulted the erythema and udema index are 0. The activity test of cinchonine niosomes for 14 days with interval observation 2 days resulted the data of hair growth in the test area was 17 - 43% longer than control area and the amount of hair in the test area 677±31,5 hair/cm , the control area 503±45,5 hair/cm . From this study show that cinchonine niosomes significantly had activity as hair growth stimulants.