DETERMINATION OF TRICLOSAN CONCETRATION IN HANDSOAP PRODUCT WITH COLORIMETRY USING DIAZOTATION METHOD
Liquid hand soap belongs to house hold product that may contain triclosan as antiseptique / disinfectant. From the safety aspect, the concentration of triclosan should not be too high because it may potentially cause skin irritation. However, this concentration should be high enough to achiev disinf...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/78951 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Liquid hand soap belongs to house hold product that may contain triclosan as antiseptique / disinfectant. From the safety aspect, the concentration of triclosan should not be too high because it may potentially cause skin irritation. However, this concentration should be high enough to achiev disinfection efectivity and or inhibiton of microbe growth when the preparation is used. The aim of this research was to obtain an analytical method for determination of triclosan applicable for routine analysis of this compound in liquid hahd soap, Triclosan which is a phenolic compound with nucleophilic properties was reacted with an electrophilic diazonium salt obtained from diazotation of 4-amino-l naphthalenesulfonic acid with nitrous acid. The reaction was an electrophilic aromatic substitution resulted in a diazo dye giving maximum absorbance at 515 nm and hence could be measured spectrophometrically. The method was validated and then applied for determination of triclosan in liquid hand soap samples with simple formulation. Measurements of triclosan standard solutions resulted in a calibration curve with a linear regression equation of y = 0.0560x — 0.2629 and r value of 0.9981. Instrumental limit of detection (LOD) and limit of quantification (LOQ) were 1.22 and 3.63 mg/L, respectively, while intra- and inter-day relative standard deviation (RSD) of the method were 0.16 and 0.26%, respectively. Percentage recoveries of the method at the spike concentrations of 500, 1000, and 3000 mg/L were 105.36, 105.36 and 103.86 0/0, respectively. The concentration of triclosan in liquid hand soap samples number 7, S, 9, were 998, 491 and 488 mg/L or equivalent to 99, 98 and 97 % of label claim. Based on overall results, it was concluded that an analytical method for determination of triclosan in liquid hand soap with simple formulation was successfully obtained and can be applied for routine determination of triclosan in this preparation.
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