OPTIMIZATION OF PRODUCTION AND CHARACTERIZATION POLYSACCHARIDES OF PORPHYRIDIUM SP.}AS ANTIDIABETIC MATERIAL

• Main Author: Ramdanawati, Liska
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78957
• Institution: Institut Teknologi Bandung

Microalgae are largely a diverse group of microorganisms comprising eukaryotic photoautotrophic protists and prokaryotic cyanobacteria. Porphyridium sp., the most abundant species of red microalgae of the divison Rhodophyta that are extensively used for polysaccharidess production. Information on in vitro studies to determine the mechanism of action of antidiabetic polysaccharides Porphyridium sp. is not clear. The purpose of this study was to obtain optimum conditions of cultivation, extraction methods, and characterization of polysaccharidess Porphyridium sp. has potential as antidiabetic material. Methods: Porphyridium sp. cultivation performed on media F/2 silicate and medium F/2 non silicate. Polysaccharidess PorphyridiuW sp. consisting of intracellular polysaccharides (IPS) and extracellular polysaccharidess (EPS). IPS extracted using heating techniques, while extracted using alcohol precipitation method and CTAB method. Polysaccharidess activity as antidiabetic material tested with a-glucosidase inhibitory activity. Selected polysaccharidess were characterized using infrared spectrometer (FTIR), high performance liquid chromatography (HPLC) and liquid chromatography mass spectroscopy (LC-MS). Results: Porphyridium sp. grew well on a F/2 non silicate medium with the growth rate of 0.2284 OD units/day. Extracellular polysaccharides (EPS) on a F/2 non silicate medium showed a-glucosidase inhibitory activity higher than intracellular polysaccharides (IPS) which was 16.10%. Extraction with CTAB method showed higher a-glucosidase inhibitory activity than alcohol precipitation method, that was 96.21 % with IC 50 values was 75400 ppm. EPS characterization results by FTIR showed characteristic functional groups of galactans structure in the area between 1660 and 1220 cm-I and the presence of sulfate ester groups in the area between 532.257-447.404 cm-I . HPLC. analysis on EPS resulting from hydrolysis using TFA indicate the presence of galactose in sample, that was 5.9 g/L. Identification of EPS resulting from hydrolisis using LC-MS showed galactose 6-sulfate molecule with fragmentation mode at m/z 261.43; 179.43; and 149.26.