LONG-TERM EFFECT OF INTERMITTENT FASTING AND CALORIE RESTRICTION ON NEUROLOGY PARAMETERS OF MICE

• Main Author: Syahida Ni'ma, Neli
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78967
• Institution: Institut Teknologi Bandung

Intermittent fasting and calorie restriction were known well for their benefits to health, especially to improve metabolic disorder and prevent neurodegenerative disease. In animals, these kinds of diet modification will increase lifespan. In spite of it, long-tenn effects of IF and CR on neurology parameters of normal mice hasn't been revealed yet. Therefore, this research was aimed to determine long-term effect of IF and CR to hedonia, depression, anxiety, aggresivity, social approach behavior, and susceptibility to PTSD in mice. In this experiment, Swiss Webster's male mice were divided to 3 group: ad libitum, intermittent fasting, and calorie restriction (65% AL's intake). Mice were treated for 16 weeks. In the end of treatment, mice were returned to AL, then tested to several selected parameters: anhedonia by sucrose preference test, depression by forced swimming test, anxiety by iigt-dark exploration test, aggresivity by resident-intruder test, and social åpproach behavior by threechamber test. They were also modeled PTSD using contextual fear conditioning method. Then, their brain tissue were observed microscopically. Compared to AL, IF groups showed reduction of sucrose intake, while CR showed increment of sucrose intake (p<0,01). Anhedonia showed in IF groups was confirmed not related to depression by forced swimming test. Even, immobility time in IF and CR group decreased compared to AL (p<0,05). In anxiety test, only one parameter was different significantly between AL and IF. It was transition number. In resident-intruder test, attack numbers in IF group was less than AL group (p<0,05). Reduction of aggresivity was not caused by deficit sociability. It was proven by three-chamber test, where time spent of IF mice in stimulus-mice chamber increased compared to AL (p<0,05). In fear extinction test (PTSD model), IF groupS showed lower freezing than AL (p<0,001). Although both IF and CR resulted in reduction of total food intake, their effects to tested neurology parameters were •different. Long-term Intermittent Fasting resulted in anhedonia; lowering depression; lowering aggresivity; improvement of social interaction; and improvement resistancy to PTSD. Long-term Calorie Restriction resulted in increasing of hedonia and lowering of depression.