ANTIMYCOBACTERIAL ACTIVITY OF TUBERCULOSIS DRUG STANDARD COMBINATION WITH ETHANOL EXTRACT OF POMEGRANATE (PUNICA GRANATUM L.) PEEL LEMONGRASS (CYMBOPHOGON CITRATUS DC. STAPF) LEAVES AND LIME (CITRUS AURONTIFOLIA CHRISTM. SWINGLE) PEEL

• Main Author: Yuned, Monalisa
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78979
• Institution: Institut Teknologi Bandung

Tuberculosis is one of the most deadly diseases in the world due to bacterial resistency. Thus, it is necessary to find an alternative drug for TB treatment. In this study, antituberculosis activity of selected medicinal plants and its interaction with standard TB drugs will be evaluated. Pomegranate (Punica granatum) peel, lemongrass (Cymbopogon citratus) leaves and lime (Citrus aurantifolia) peel was extracted using 96% ethanol. Antituberculosis activity of the plants against Mycobacterium tuberculosis H37Rv sensitive, Isoniazid-Ethambutol (HE) resistant and Streptomycin-Rifampicin (SR) resistant was tested using Lowenstein-Jensen (LJ) media. To study the interaction with standard drug, both standard drug and choosen plants were added in the LJ media. Number of colony were counted as a parameter for antituberculosis activity. The results obtained in the phytochemical screening, all of the extracts and crude plants contained alkaloids and flavonoids. C.aurantifolia at dose of 10, 25, 100 gg/mL, P.granatum at dose of 10, 25, 50 gg/mL and C.citratus at dose of 10, 25, 50, 100 gg/mL inhibited the growth of sensitive M.tuberculosis more than 50%. While against M.tuberculosis SR resistant 10-5 McFarland, only P.granatum at dose of 100 gg/mL could inhibit the growth more than 50%. Furthermore, C.aurantifolia at dose of 25 and 100 gg/mL, P.granatum at dose of 25 ug/mL and C.citratus at dose of 25 and 50 pg/mL showed an antibacterial activity against M. tuberculosis HE resistant. The interaction between selected extract with standard drug against M.tuberculosis resistant 10-5 McFarland showed that P.granatum and C.citratus gave an increase antibacterial activity when it was combined with streptomycin, P.granatum and C.aurantifolia gave an increase antibacterial activity when it was combined with ethambutol. The interaction between isoniazid with C.citratus and rifampisin with C.aurantifolia gave a decrease antibacterial activity. The conclusion of this study is ethanolic extract of P.granaium at dose of 100 ug/mL is potential to be develop as an alternative drug for4B treatment and gave an increase antibacterial activity when it was combined with streptomycin and ethambutol against M. tuberculosis resistant 10-5 McFarland.