THE EFFECT OF ROSEMARY LEAVES EXTRACT, GINGER, JAVA TURMERIC, AND CURCUMIN TOWARD KETOCONAZOLE AS AN ANTIFUNGAL ACTIVITY

• Main Author: Eldina Pattalia, Annisa
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78981
• Institution: Institut Teknologi Bandung

Dermatological fungal infections in Indonesia has high prevalence because its tropic climate and high humidity- Dermatophytosis is a common superficial fungal infection, caused by dermathophyta. Therapy for dermathophytosis came from conventional antifungi or new coumpounds from natural source. However, the effectiveness of both therapy did not work well. This research is to test interaction in combination between ethanolic extract of rosemary leaves, ginger, java turmeric, curcumin and ketoconazole against Microsporum vpseum and Trichophyton mentagrophytes. This research is to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) has been done by checkerboard method of CLSI, while to determine the combination effect (synergistic, additive, or antagonism) from compound with ketoconazole has been done by checkerboard method and the paper strips diffusion technique. Curcumin showed the antifungal activity with MC value, against Microsporum gvpseum are 1024 gg/mL and Trichophyton mentagrophytes with BNC value are 128 pg/mL The MFC of curcumin against Microsporum gypseum are >2048 gg/mL and Trichophyton mentagrophytes with MFC value are 512 gg/mL. Synergistic interaction against Microsporum vpseum and Trichophyton mentagrophytes was obtained from the combination of ginger extract with ketoconazole. Additive interaction against and Microsporum gypseum and Trichophyton mentagrophytes was obtained from the combination of rosmary leaves extract, java turmeric, and curcumin, respectively the combination with ketoconazole. In conclusion, combination of ginger ethanolic extract with ketoconazole has increase an antifungal effect with low concentration if used both therapy.