AKTIVITAS PENGHAMBATAN LIPOKSIGENASE (LOX) OLEH EKSTRAK TUMBUHAN SUKU ZINGIBERACEAE DAN ISOLASI METABOLIT SEKUNDER TUMBUHAN TERPILIH
Lipoxygenase (LOX) is an enzyme essential for the formation of leukotriene, an endogenous substance involved in inflammatory pathological conditions including asthma and rheumatoid arthritis. Plants which belong to Zingiberaceae family have been empirically used as drug in Indonesia. This study was...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/79029 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Lipoxygenase (LOX) is an enzyme essential for the formation of leukotriene, an endogenous substance involved in inflammatory pathological conditions including asthma and rheumatoid arthritis. Plants which belong to Zingiberaceae family have been empirically used as drug in Indonesia. This study was aimed to screen the LOX inhibiting activity of 18 plants from to Zingiberaceae family and to isolate secondary metabolites with the highest activity. Extraction was carried out by maceration using 95% ethanol. LOX inhibiting activity was determined by comparing the absorbance of hydroperoxyeicosatetranoate (HPETE) formed in the presence or absence of the extracts. Fractionation was done to the active extract using liquie-liquid extraction with n„hexane and ethylacetate. The active n-hexane fraction was then separated and purified using vacuum liquid chromatograhpy, radial chromatography, and washing. The level of purity of isolate was controlled by two dimensional thin layer chromatography and melting range test. Results showed that ethanol extract of Curcuma zedoaria at 100 ppm had the highest inhibiting activity compared to the other extracts with with a value of 54.8 %. nhexane fraction of Curcuma zedoaria showed the most potent activity with IC50 of 103.8 ppm compared to that of ethylacetate and aqueous fractions, with respective IC50 of 406.05 ppm and 192.84 ppm. The IC50 of the reference substance baicailein was 6.59 ppm. In this study, a compounds had been isolated from Curcuma zedoaria which took the form of orange-colored amorphous solid. Taken together, results of the present study show that ethanol extract of Curcuma zedoaria has the highest LOX inhibiting activity with a value of 54.58 %, and the active isolate belongs to curcuminoid class.
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