AKTIVITAS PENGHAMBATAN ANGIOTENSIN CONVERTING ENZYME (ACE) BEBERAPA EKSTRAK TUMBUHAN INDONESIA DAN ISOLASI SENYAWA TUMBUHAN TERPILIHÂ
Angiotensin Converting Enzyme (ACE) is known to catalyze the conversion of angiotensin I to angiotensin Il, if ACE converts angiotensin I excessively, it will lead to the increase blood pressure. One way to prevent the increasing in blood pressure by inhibiting ACE. This study aimed to determine the...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/79033 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Angiotensin Converting Enzyme (ACE) is known to catalyze the conversion of angiotensin I to angiotensin Il, if ACE converts angiotensin I excessively, it will lead to the increase blood pressure. One way to prevent the increasing in blood pressure by inhibiting ACE. This study aimed to determine the ACE inhibitory activity of ethanolic extracts of eighteen species of plants and isolating compounds from those plants that had the highest ACE inhibitory activity. Measurement of ACE inhibitory activity using the method of Chusman and Cheung was used to measure. The principle of that measuring the absorbance of hippuric acid produced by the reaction of the substrate with the ACE and the test materials. Extract that had the highest ACE inhibitory activity was continued to further process by liquid-liquid extraction, vacuum liquid chromatography, radial chromatography and purification. The results showed that extract with more than 50 % ACE inhibitory activity were ethanolic extracts of Averrhoa bilimbi L. (71.49 %), Morinda citrifolia L. (66.64 %), Orthosiphon stamineus Benth. (55.41 %), Syzygium polyanthum (Wight) Walp. (53,37 %), Solanum indicum Linn. (53.24 %), Nasturtium officinale R. Br. (51.94 %) and Zea mays L. (50.44%). Ethanolic extract of Averrhoa bilimbi L. had the highest ACE inhibitory activity among the others extracts. The IC50 value of the ethanolic extract of Averrhoa bilimbi L. was 15.03 ppm, and IC50 captopril was 1.63 ppm. Ethanolic extract of Averrhoa bilimbi L. was fractionated by liquid-liquid extraction method to obtain n-hexane fraction, ethyl acetate fraction and water fraction. N-hexane fraction (77.29 %) showed the highest ACE inhibitory activity followed by water fraction (70.63 %) and ethyl acetate fraction (65,64 %). Isolation process obtained isolate A which showed fluorescent at 366 nm and gave a red-violet color with sulfuric acid 10 % in methanol. Isolate A was sprayed by Liebermann-Burchard reagent and gave brownish-red color. Based on those results the ethanolic extract of Averrhoa bilimbi L. had the highest ACE inhibitory activity (71 ,49 %) and isolate A was identified as steroid compound.
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