EFFECT OF BENZIL AMINO PURINE AND SUCROSE ON DEVELOPMENT OF MICRORHIZOME AND PRODUCTION OF XANTHORRIZOL IN TISSUE CULTURE OF TEMULAWAK CURCUMA XANTHORRIZA ROXB.

• Main Author: Perdana, Farid
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/79037
• Institution: Institut Teknologi Bandung

Background and aims: Temulawak (Curcuma xanthorriza Roxb.) is one of The Indonesian plants belonging to Zingiberaceae. One of the constituent, xanth0ffhizol is a unique marker for C. xanthorrhiza and thus it's presence differentiates this plant from the others and mainly produced in rhizome. Nevertheless, the xanthorrhizol levels in tissue culture has not been reported. Therefore, the objective of this study was to know the influence of different combination-concentration of BAP (benzyl amino purine) and sucrose on microrhizome fonnation and xanthorrhizol levels in culture of C. xanthorriza. Method: The rhizome of C. xanthorriza was sterilized and grown up on pre-culture medium (Murashige and Skoog) for one week, and then grown up on microrhizome induction and formation medium suplemented with BAP (1, 2, 3 mg/L) and sucrose (6, 9, 12 g/L). The height of shoots, length of leaves and weight of rhizoms were measured at four, six and eight weeks after planted. The xanthorrhizol level wes analyzed after two months subculture using TLC densitometry. Results: Media S7 (3 mg/L BAP + 60 g/L sucrose) induced the highest number of shoots, media S6 (2mg/L BAP + 12 [L sucrose) induced the highest dry weight of microrhizome, media S7 (3 mg/L BAP + 6 g/L sucrose) gave the highest shoot and xanthorrhizol level at 0,25% and the wild rhizome gave the xanthorrhizol level at 0,04 %. Conclusion : Microrhizome culture yielded xanthorrhizol five times highest than the wild.