SINTESIS SENYAWA TURUNAN 1-BENZOIL-3-METIL TIOUREA SEBAGAI KANDIDAT SENYAWA ANTIKANKER

• Main Author: Sutanto, Fandi
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/79070
• Institution: Institut Teknologi Bandung

Cancer is a disease characterized by the occurrence of a failure in the regulation mechanism of cell multiplication. Chemotherapy, the common method for cancer thera y, has many side effects. Several thiourea derivatives are known for their anticancer activities, such as 2-[3-(2-Methyl-benzoyl)-thioureido]-acetic acid and 2-[3-(4-Methyl-benzoyl)-thioureido]-acetic acid that fight MCF-7, breast cancer's cell. I-Methyl-2-thiourea was reacted with benzoyl chloride derivatives in tetrahydrofuran and triethylamine as the reaction medium, and the mixture has to be heated. The organic phase was evacuated under vacuum to obtain the product and then was purified using recrystallization method with ethanol. The result will go through purity test using melting-point-range method and thin layer chromatography method and then will be identified and characterized using UV/Vis spectrophotometry, infrared (IR) spectroscopy, and nuclear magnetic resonance spectroscopy (NMR). Melting-point-range and thin layer chromatography as purity test methods indicate that those synthesized compounds were pure. Identification and characterization using UV/Vis spectrophotometry showed maximum absorbance at wavelength 245, 255, and 260 nm, indicates the benzene group, infrared spectroscopy showed transmittance at wavenumber range 3400 — 3000 cm-I , indicates the amine group, wavenumber range 1680 — 1630 cm indicates the carbonyl group, and wavenumber range 3100 — 3000 cm-I and 1600 — 1500 cm-I , indicates the benzene group, I H-NMR and BC-NMR showed that there are benzene, carbonyl, amine, and methyl groups shown by the peaks in specific chemical shift. I-Benzyl-3-methyl thiourea derivatives can be synthesized by reacting I-methyl-2-thiourea with benzoyl chloride derivative by heating to dissolve the mixture and tetrahydrofuran and triethyiamine- as the reaction medium to neutralize the acid formed. Identification and characterization using spectrophotometry UV/Vis showed the existence of benzene group, infrared spectroscopy showed the existence of carbonyl and amine groups, while H-NMR and B C-NMR showed the existence benzene, carbonyl, amine, and methyl groups of I-benzoyl-3-methyl thiourea derivatives.