BACTERIOPHAGE APPLICATION TO CONTROL ENTEROBACTER HORMAECHEI INFECTION AND TO INCREASE SURVIVAL RATE AND GROWTH OF WHITE-LEG SHRIMP (LITOPENAEUS VANNAMEI)
Bacterial infection still majorly threatens white shrimp (Litopenaeus vannamei) production. Four Enterobacter hormaechei strains were isolated from shrimp gut and were proven to possess pathogenecity towards L. vannamei post-larvae, causing mortality and slow growth. To treat the infection, phage th...
Saved in:
| Main Author: | |
|---|---|
| Format: | Theses |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/80022 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Bacterial infection still majorly threatens white shrimp (Litopenaeus vannamei) production. Four Enterobacter hormaechei strains were isolated from shrimp gut and were proven to possess pathogenecity towards L. vannamei post-larvae, causing mortality and slow growth. To treat the infection, phage therapy developed using four bacteriophages isolated from shrimp rearing and disposal water was seen potential. This research aims to assess the effect of four phages and their cocktai to survival rate and growth of L. vannamei post-larvae infected by E. hormaechei strains under proper dosage. Dosage was determined by testing phages at different multiplicity of infections (MOIs) to cocktail culture of E. hormaechei strains. Performance test was conducted by adding phages at optimal dose to post-larvae culture previously infected by E. hormaechei strains for 10 days. Upon harvesting, survival rate (SR), mean body weight (MBW), and mean body length (MBL) were measured. Result showed that phages were able to effectively supress the growth of E. hormaechei cocktail at MOI = 0.01. When being administered to pre-infected post-larvae, all phage treatments except ?TC minimized post-larvae mortality rate. ?BT and ?BC performance were effective against E. hormaechei spp. 05 (SR = 91.11±3.84%) and spp. 07 (SR = 91.11±10.18%) respectively, comparable to chloramphenicol (SR05 = 100±0.00%, SR07 = 97.78±3.85%). Although all phage treatments showed less weight reduction in post-larvae compared to control, no significant effect was observed to shortened length. This study shows the possibility of phages to be used in phage therapy treating E. hormaechei infection against L. vannamei post-larvae. |
|---|