PRELIMINARY DESIGN OF LIPID PRODUCTION SYSTEM FROM CULTURED SPIROGYA SP. ON ALGAL TURF SCRUBBER AND PRODUCTION OF OTHER BIOPRODUCTS BY APPLYING BIOREFINERY CONCEPT
One of macroalgae species, Spirogyra sp., has promising potential as an alternative resource of lipid. In some freshwater green algae species, addition of inorganic carbon sources in culture medium has been shown to promote lipid synthesis in algal cells. The aim of this study was to increase lip...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/80082 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | One of macroalgae species, Spirogyra sp., has promising potential as an
alternative resource of lipid. In some freshwater green algae species, addition of
inorganic carbon sources in culture medium has been shown to promote lipid
synthesis in algal cells. The aim of this study was to increase lipid accumulation
in freshwater macroalgae Spirogyra sp. by varying the C:N ratio through the
addition of sodium bicarbonate as an inorganic carbon source. The addition of
sodium bicarbonate was started from the beginning of the cultivation period to
see how it affected algal biomass growth. Spirogyra sp. was grown in modified
Bold Basal Medium with sodium bicarbonate added at 2:1, 4:1 and 6:1 C:N
ratios. Cultivation was carried out for 8 days with triplicate, and data on biomass
and lipid yields was collected every two days. The logistic model was used to
estimate the biomass growth parameter values, whereas the Luedeking-piret
product formation model was used to predict lipid production by estimating ? and
? parameters. The experimental result showed that as the C:N ratio in the medium
increases, the culture’s maximum specific growth rate increases but the biomass
yield decreased. The highest biomass yield was achieved with a 2:1 C:N ratio i.e.
404.3 mg L-1 on the 8th day of cultivation. The addition of sodium bicarbonate
treatment was also proven to increase lipid accumulation in all variations, with
the highest lipid yields at a 2:1 C:N ratio i.e. 79.51 mg g-1 on the 2nd day of
cultivation. According to the estimation of lipid productivity based on the
modeling of biomass growth kinetics and lipid production, the maximum value
was reached by adding sodium bicarbonate at 4:1 C:N ratio with 6.99 mg g-1day-
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