BIOSYNTHESIS OF COPPER NANOPARTICLES USING PHYSALIS ANGULATA LEAVES EXTRACT AS REDUCTOR ALONG WITH ITS ANTIBACTERIAL AND ANTIBIOCORROSION ACTIVITY

• Main Author: Faiz Rizky, Muhammad
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/80491
• Institution: Institut Teknologi Bandung

Copper nanoparticle, a material that can act as an effective biocide, are toxic to microbes but safe for animal and human cells. This material can be synthesized using biological methods that are relatively simple, cost efficient, and also environmentally friendly. One of the biological methods used for synthesis is by utilizing a plant called ciplukan. Ciplukan (Physalis angulata) is a plant that is easy to obtain and cultivate as it can grow both in high and lowlands. The leaves of this plant can be used as a reducing agent in the biosynthesis process of copper nanoparticles because of the secondary metabolites contained therein. More than 75% of cases of corrosion in oil wells and more than 50% of submarine pipe and cable failures are caused by corrosion-causing bacteria. It is expected that the copper nanoparticles produced from the biosynthesis process can act as a biocide against corrosion-causing bacteria and be the key in solving the corrosion problem that is commonly found. In this study, precursors in the form of CuSO4 and ciplukan leaf extract were used as reducing agents in the biosynthesis process. The parameters of precursor concentration, extract volume, time and reaction temperature were varied to determine the optimum parameters in the biosynthesis process. Characterization of biosynthesized nanoparticles were done using UV-Visual Spectrophotometer (UVVis), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscope-Energy Dispersive X-ray Spectroscopy (SEM-EDX), and Atomic Absorption Spectroscopy (AAS). The copper nanoparticles were then tested for its antibacterial potential against gram-negative bacteria such as Escherichia coli and gram-positive bacteria such as Staphylococcus saprophyticus with varying concentrations. The anti-biocorrosion test was carried out using API 5L X52 steel in seawater media containing the sulfate reducing bacteria Citrobacter freundii. Electrochemical test using a Potentiostat was carried out on immersion day 0, 5, and 10 to determine the effect of copper nanoparticles incorporation on the biocorrosion events that occurred. The results of this study shows that the optimum concentration was 20 mM, the optimum extract volume was 1 mL, the optimum reaction time was 30 minutes, and the optimum reaction temperature was 70?. The experiments that have been carried out prove that with a greater concentration of copper nanoparticles, the ability to inhibit bacterial growth is also eminent. The copper nanoparticles produced from the utilization of ciplukan leaves function as a biocide against corrosion-causing bacteria as it can reduce the potential of corrosion and inhibit the rate of corrosion if the corrosion has been initiated.