THE EFFECT OF FUNCTIONAL FEED FROM PLEUROTUS OSTREATUS CULTIVATION WASTE ON CULTURE PERFORMANCE, COMMUNITY LEVEL PHYSIOLOGICAL PROFILING, HISTOLOGY PROFILE OF HEPATOPANCREAS, AND SURVIVABILITY OF WHITE LEG SHRIMP LITOPENAEUS VANNAMEI CHALLENGED TO VIBRIO PARAHAEMOLYTICUS INFECTION IN ZERO WATER DISCHARGE (ZWD) SYSTEM

• Main Author: Jannu Aryanto S. P., Faizal
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/80848
• Institution: Institut Teknologi Bandung

Abstract Utilization of functional feed through supplementation of bioactive compounds accompanied by a good water quality management system is one of the most important factors for the success story of aquaculture industry. Pleurotus ostreatus mushroom polysaccharides, are known to have immunostimulating effects in feed supplementation for shrimp. In P. ostreatus cultivation industry, spent mushroom substrate (SMS) containing P. ostreatus mycelia was used as functional feed for shrimp due to its high content of polysaccharides. The SMS containing high polysaccharides further extracted, dried, and powdered (SMS extract) before polysaccharides content was measured. This study examined the effectiveness of the extract as a feed supplement at 1% and 0.5% (w/w) in the cultivation of white shrimp Litopaneus vannamei for 80 days in Zero Water Discharge (ZWD) system. The extraction process yielded 0.198 g of polysaccharide per gram extract. Administration of the extract did not impact the ZWD system or the aquatic bacterial community. Feeding with 1% extract for 40 days damaged hepatopancreatic cell integrity, reducing white shrimp survival at 80 days. The survival rate against the pathogen was lowest in the 1% extract at 11.11%, followed by the control group at 30.56%, and the 0.5% extract at 43.24%. Feeding 0.5% extract increased shrimp weight to 17 g compared to the control of 16.32 g. Important substrates that indicated changes in the physiological profile of the gut microbiota included ketobutyric acid, D-mannitol, L-threonine, glycogen, L-serine, L-arginine, Tween 40 and pyruvic acid methyl ester. Thus, providing 0.5% extract as a supplement and using the ZWD system can increase shrimp growth and survival without affecting system performance. This study can be used as a reference to explore further mechanisms of gut microbiota and their interactions with shrimp physiological conditions.