DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR DETERMINING LUTEOLIN CONTENT IN TEMPUYUNG (SONCHUS ARVENSIS L.) LEAVES EXTRACT AND NANO EXTRACT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

• Main Author: Nataprasaja, Felita
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82412
• Institution: Institut Teknologi Bandung

Dyslipidemia is a lipid metabolism disorder that can lead to cardiovascular disease complications. Conventional drugs as dyslipidemia therapy have various side effects so alternative therapies are also widely used. Sonchus arvensis L. leaves are the example of natural ingredient that can be used as an alternative therapy for dyslipidemia. To ensure the quality of the S. arvensis leaves extract and nanoparticle of S. arvensis leaves extract, a validated analytical method is needed to determine the level of luteolin contained therein. Thus, this research was carried out to develop a method for analyzing luteolin content in S. arvensis leaves extract and nanoparticle of S. arvensis leaves extract using high performance liquid chromatography (HPLC). Based on the optimization results, the optimum chromatography system was using ZORBAX Eclipse XDB-C18 with guard column as the stationary phase, 0.1% formic acid in 10% acetonitrile and acetonitrile as the mobile phase with gradient elution, flow rate of 1 ml/min, and UV-Visible detector with detection wavelength at 350 nm. The method validation results showed good selectivity with a resolution value of 5.8. The method showed good linearity in the range of 1 - 8 ?g/ml with r = 0.9995 and Vx0 = 1.7%. The detection and quantitation limits were 0.252 and 0.765 ?g/ml. The recovery for S. arvensis leaves extract and nanoparticle of S. arvensis leaves extract were 98.97 - 102.27 % and 98.27 - 103.07 %. The intraday and interday precision met the requirements and the method has been proven to have good robustness. Determination of luteolin content using the validated method showed that the luteolin content in S. arvensis leaves extract and nanoparticle of S. arvensis leaves extract were 310 ± 9.82 and 370 ± 4.86 ?g/g sample.